抗阻运动诱导骨骼肌释放鸢尾素并改善2型糖尿病大鼠骨髓内皮祖细胞功能  

Resistance exercise induces release of irisin from skeletal muscle and improves function of bone marrow endothelial progenitor cells in type 2 diabetic rats

作　　者：寇猛 胡好 张勇 陈亚东 KOU Meng;HU Hao;ZHANG Yong;CHEN Yadong(College of Physical Education,Anhui Polytechnic University,Wuhu 241000,China;College of Sport Science,Hefei Normal University,Hefei 230061,China)

机构地区：[1]安徽工程大学体育学院,安徽芜湖241000 [2]合肥师范学院体育科学学院,安徽合肥230061

出　　处：《中国病理生理杂志》2025年第1期19-27,共9页Chinese Journal of Pathophysiology

基　　金：安徽省质量工程重点研究项目(No.2021xsxxkc250)。

摘　　要：目的:观察抗阻运动对2型糖尿病大鼠模型内皮祖细胞功能的影响并探讨肌因子鸢尾素在其间的作用机制。方法:40只6周龄健康雄性SD大鼠,取10只作为对照组,其余用高脂高糖饲料喂养联合链脲佐菌素腹腔注射进行糖尿病造模后随机分为模型组和模型运动组,每组15只。对照组和模型组动物于鼠笼内安静饲养,模型运动组进行12周(3次/周)尾部负重爬梯训练。末次训练后72 h,分离血浆、腓肠肌和股骨,测定各组织鸢尾素含量以及腓肠肌和股骨含纤连蛋白Ⅲ型结构域蛋白5(FNDC5)mRNA和蛋白表达量;分离培养骨髓内皮祖细胞,经外源性鸢尾素处理48 h后,利用CCK-8法、黏附实验分别检测细胞活力和黏附能力,通过裸鼠颈动脉损伤模型检测内皮修复能力,Western blot法检测磷脂酰肌醇3-激酶(PI3-K)/Akt/内皮型一氧化氮合酶(eNOS)信号通路蛋白的表达量。结果:(1)在体实验:与对照组比较,模型组血浆、腓肠肌和股骨鸢尾素含量以及腓肠肌FNDC5 mRNA和蛋白表达量降低(P<0.05);与模型组比较,模型运动组血浆、腓肠肌和股骨鸢尾素含量以及腓肠肌FNDC5 mRNA和蛋白表达量升高(P<0.05);各组股骨FNDC5 mRNA和蛋白表达无显著性变化(P>0.05)。(2)细胞实验:①内皮祖细胞未经鸢尾素处理:与对照组比较,模型组内皮祖细胞活力、黏附功能以及内皮损伤修复能力降低(P<0.05),PI3-K、Akt、eNOS蛋白表达下调(P<0.05);与模型组比较,模型运动组内皮祖细胞功能改善(P<0.05),PI3-K、Akt、eNOS蛋白表达上调(P<0.05)。②内皮祖细胞经鸢尾素处理:与未经鸢尾素处理组比较,补充鸢尾素后模型组和模型运动组内皮祖细胞功能提升(P<0.05),PI3-K、Akt、eNOS蛋白表达显著增加(P<0.05)。结论:规律抗阻运动能够改善2型糖尿病大鼠内皮祖细胞功能,其机制可能与骨骼肌释放的肌因子鸢尾素调控PI3-K/Akt/eNOS信号通路有关。AIM:To investigate the effect of resistance exercise on the function of endothelial progenitor cells(EPCs)in type 2 diabetic rats and explore the mechanism of myokine irisin in this process.METHODS:Forty 6-weekold healthy male SD rats were selected,ten of which were used as control group,and the rest were fed with high-fat and high-sugar diet combined with streptozotocin injection for diabetic modeling.The rats were randomly divided into model group and model exercise group,with 15 rats in each group.The animals in the control and model groups were kept quietly,and those of model exercise group underwent tail-loaded ladder climbing training for 12 weeks(3 times/week).72 hours after the last training,plasma,gastrocnemius and femur were separated.The irisin content of each tissue and mRNA as well as protein expression of fibronectin typeⅢdomain-containing protein 5(FNDC5)in the gastrocnemius and femur were measured.Bone marrow endothelial progenitor cells were isolated and cultivated,and then treated with exogenous irisin for 48 hours.The cell viability and adhesion function of endothelial progenitor cells were detected by CCK-8 and adhesion assay,respectively.The endothelial repair ability was detected by carotid artery injury model in nude mice.The protein expression of phosphatidylinositol 3-kinase(PI3-K)/Akt/endothelial nitric oxide synthase(eNOS)signaling pathway was detected by Western blot.RESULTS:(1)In vivo experiment:compared with the control group,the model group showed a decrease in plasma,gastrocnemius muscle,and femoral irisin content,as well as a decrease in gastrocnemius muscle FNDC5 mRNA and protein expression levels(P<0.05).Compared with the model group,the levels of irisin in plasma,gastrocnemius muscle and femur,as well as the expression of FNDC5 mRNA and protein in gastrocnemius muscle,increased in the model exercise group(P<0.05).There was no significant change in the expression of FNDC5 mRNA and protein in the femurs of each group(P<0.05).(2)Cell experiment:①endothelial progenitor cells wi

关 键 词：抗阻运动 糖尿病 骨骼肌 鸢尾素 内皮祖细胞 

分 类 号：R455[医药卫生—运动医学] R337[医药卫生—治疗学] R587.1[医药卫生—临床医学]