沙蓬粗提物对陇南花椒黑胫病病原菌抑制作用研究  

Study on the inhibitory effect of crude extract of Agriophyllum squarrosum on the pathogen of blackshank disease of Zanthoxylum bungeanum in Longnan

作　　者：史梁 钱朝菊 范兴科 燕霞 曹永红 张洪 陆欣 马小飞 SHI Liang;QIAN Chaoju;FAN Xingke;YAN Xia;CAO Yonghong;ZHANG Hong;LU Xin;MA Xiaofei(Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China;Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China;University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Inland River Ecohydrology,Cold and Arid Regions Environmental and Engineering Research,Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China;Longnan Academy of Non-wood Forest,Longnan 742100,China;Gansu Yulinlong Low Carbon Energy Saving Technology Co.,Ltd.,Lanzhou 730000,China)

机构地区：[1]中国科学院西北生态环境资源研究院干旱区生态安全与可持续发展重点实验室,兰州730000 [2]中国科学院西北生态环境资源研究院甘肃省寒区旱区逆境生理与生态重点实验室,兰州730000 [3]中国科学院大学,北京100049 [4]中国科学院西北生态环境资源研究院中国科学院内陆河流域生态水文重点实验室,兰州730000 [5]陇南市经济林研究院,陇南742100 [6]甘肃育霖陇低碳节能科技有限公司,兰州730000

出　　处：《生物学杂志》2025年第1期61-66,共6页Journal of Biology

基　　金：中国科学院战略生物资源计划项目(KFJ-BRP-007-015);国家自然科学基金项目(32271695);甘肃省省级引导科技创新发展专项(Y939BD1001);兰州市人才创新创业项目(2021-RC-76);兰州市青年科技人才创新项目(2023-QN-140)。

摘　　要：为了明确病原菌,采用形态学和ITS测序的方法对陇南地区花椒黑胫病病原菌进行分离和鉴定,从多株陇南花椒的黑胫病病灶部位分离纯化得到13种不同菌落形态特征的菌株,经过寄主回接,科赫法则验证,首次明确陇南地区花椒黑胫病病原菌为一种极细链格孢菌。通过梯度生长抑制测定法评估沙蓬粗提物对该极细链格孢菌的抑制效果,发现8 mg/mL的沙蓬粗提物对极细链格孢菌的抑制效果最佳,抑制率达到70.18%。研究结果不仅说明花椒黑胫病产生的机制复杂多样,同时说明沙生药用植物沙蓬的粗提物作为植物源杀菌剂在重要经济林作物主要病害的生物防治中具有广阔前景,拓展了沙蓬在我国特色生态农业中的应用方向。In order to clarify the pathogenic fungi,morphological and ITS sequencing methods were used to isolate and identify the pathogenic fungi of black shank disease on Zanthoxylum bungeanum in Longnan area.13 strains with different colony morphological characteristics were isolated and purified from the black shank disease lesions of multiple Longnan Z.bungeanum strains.After host tieback and Koch’s rule verification,it was clarified for the first time that the causative agent of Z.bungeanum black shank in Longnan was a type of Alternaria tenuissima.The inhibitory effect of the crude extract of Agriophyllum squarrosum against A.tenuissima was evaluated by gradient growth inhibition assay.It was found that the crude extract of 8 mg/mL of A.squarrosum had the best inhibitory effect on A.tenuissima and the inhibition rate reached at 70.18%.The research case not only illustrated the complex and diverse mechanisms of the occurrence of black shank in Z.bungeanum,but also supported that the crude extract of the psammophila medicinal plant A.squarrosum had broad prospects as a botanical fungicide in the biological control of major diseases of important economic forest crops,and it expanded the application direction of A.squarrosum in China’s characteristic ecological agriculture.

关 键 词：沙蓬粗提物 花椒黑胫病 病原菌 极细链格孢菌 植物源杀菌剂 

分 类 号：Q948.122.3[生物学—植物学] S435.73[农业科学—农业昆虫与害虫防治]