基于TRM细胞探讨刺络放血对咪喹莫特诱导的复发型银屑病小鼠的影响及作用机制  

Exploration on Effects and Mechanism of Bloodletting Puncture Therapy on Mice with Imiquimod-Induced Recurrent Psoriasis Based on TRM Cells Changes

作　　者：吴明明[1] 问雅琦 王建青[1] 郭士洋 WU Mingming;WEN Yaqi;WANG Jianqing;GUO Shiyang(Shanxi Hospital of Chinese Medicine,Taiyuan,Shanxi 030012;Shanxi Academy of Chinese Medicine,Taiyuan,Shanxi 030012;Shanxi University of Chinese Medicine,Taiyuan,Shanxi 030024)

机构地区：[1]山西省中医院,山西太原030012 [2]山西省中医药研究院,山西太原030012 [3]山西中医药大学,山西太原030024

出　　处：《中国中医药科技》2025年第1期5-10,53,共7页Chinese Journal of Traditional Medical Science and Technology

基　　金：山西省卫生健康委员会科研项目(2022100)。

摘　　要：目的:基于组织常驻记忆性T细胞(tissue resident memory T cells,T_(RM))探讨刺络放血对咪喹莫特诱导的复发型银屑病小鼠的影响及作用机制。方法:采用背部涂抹咪喹莫特乳膏(Imiquimod,IMQ)二次诱导的方法建立复发型银屑病模型。将实验随机分为分为正常对照组、模型组、刺络放血组、阳性对照组4组,每组6只C57BL/6小鼠,造模后刺络放血组小鼠在背部皮损夹脊穴区域进行刺络放血,阳性对照组小鼠在背部皮损区域均匀涂抹卡泊三醇软膏,正常对照组、模型组小鼠只抓取,不治疗,共治疗6 d。记录各组小鼠背部皮肤表现并进行银屑病面积与严重程度指数(PASI)评分;取各组小鼠背部皮肤HE染色法观察皮肤组织形态表现并行表皮厚度测量,免疫组化及流式细胞术检测各组小鼠背部皮肤组织CD_(8)^(+)T_(RM)与CD_(69)表达。结果:与正常对照组相比,模型组小鼠明显可见红斑、鳞屑、皮肤增厚等表现,PASI评分显著升高,病理切片可见角化过度、炎性细胞浸润、局部出血及表皮层明显增厚(P<0.01);与模型组相比,刺络放血组小鼠红斑、鳞屑、皮肤增厚等表现均减轻,PASI评分降低,病理切片角化过度、炎性细胞浸润、局部出血程度减轻,表皮层增厚减轻(P<0.05);刺络放血组与阳性对照组比较,差异无统计学意义(P>0.05)。与正常对照组相比,模型组小鼠背部皮肤组织CD_(8)^(+)T_(RM)及CD_(69)阳性细胞数显著增多(P<0.01);与模型组相比,刺络放血组小鼠背部皮肤组织CD_(8)^(+)T_(RM)及CD_(69)阳性细胞数有所减少(P<0.05);刺络放血组与阳性对照组比较,差异无统计学意义(P>0.05)。结论:刺络放血可能通过抑制机体T_(RM)细胞的表达起到改善银屑病的作用。Objective:To explore the effects and mechanism of Bloodletting puncture therapy in mice model of imiquimod-induced recurrent psoriasis based on the changes of T_(RM)cells.Methods:The recurrent psoriasis model was established by applying Imiquimod(IMQ)on the back.The experiment was randomly divided into 4 groups,including normal control group,model group,Bloodletting group,and positive control group,with 6 C57BL/6 mice in each group.Mice in the Bloodletting group were treated with bloodletting puncture therapy on the skin of the Huatuo Jiaji points of mice,mice in the positive control group were administrated with Cappotriol on the skin of the back of mice,the normal and the model group were not treated.The course of treatment was 6 days.Record the back skin manifestations of mice and the Psoriasis area and Severity Index(PASI)score were recorded.The back skin of the mice in each group was collected for detection.Skin tissue was stained by HE to observe pathological findings and measure epidermal thickness,the expression of CD_(8)^(+)T_(RM)and CD_(69)was determined by immunohistochemostry and flow cytometry.Results:Compared to the normal control group,the model group of mice showed significant erythema,scales,skin thickening and other manifestations,the PASI scores were significantly increased,and hyperkeratosis,inflammatory cell infiltration,local hemorrhage,and significant thickening of the epidermal layer were observed in the pathological sections(P<0.01);compared to the model group,the manifestations of erythema,scales and skin thickening in the Bloodletting group were effectively reduced,PASI score was effectively reduced,with re-duced excessive keratosis,inflammatory cell infiltration and local bleeding,and decreased epidermal layer thick-ness(P<0.05).There was no statistically significant difference between Bloodletting group and positive control group(P>0.05).Compared with the normal control group,the number of CD_(8)^(+)T_(RM)and CD_(69)positive cells was significantly increased in the model group,and the

关 键 词：复发型银屑病 刺络放血 病理学 组织常驻记忆性T细胞 小鼠 

分 类 号：R75[医药卫生—皮肤病学与性病学]