香草扶正合剂对Lewis肺癌小鼠肿瘤组织能量代谢及PD-L2、RGMb表达的影响  

Effects of Xiangcao Fuzheng Mixture(香草扶正合剂)on Energy Metabolism and Expressions of PD-L2 and RGMb in Tumor Tissues of Lewis Lung Cancer Mice

作　　者：程权 傅华洲[1] CHENG Quan;FU Huazhou(Hangzhou First People’s Hospital,Hangzhou,Zhejiang 310006)

机构地区：[1]杭州市第一人民医院,浙江杭州310006

出　　处：《中国中医药科技》2025年第1期20-25,共6页Chinese Journal of Traditional Medical Science and Technology

基　　金：国家中医药管理局第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号);浙江省医药卫生科技计划项目(2024KY1323);浙江省中医药防治重大疾病攻关计划项目(2018ZY009);浙江省名老中医专家传承工作室建设项目(GZS2020029)。

摘　　要：目的:探讨香草扶正合剂(Xiangcao Fuzheng Mixture,XCFZ)对Lewis肺癌小鼠的抑瘤效应及机制。方法:采用Lewis肺癌细胞株培养移植法建立肺癌荷瘤小鼠模型,随机分为模型组,顺铂(DDP)组,XCFZ低、中、高剂量组,联合组,每组10只;造模第2天开始XCFZ低、中、高剂量组分别予9.3、18.6、37.2 g/kg XCFZ灌胃;顺铂组以5 mg/kg顺铂溶液隔天腹腔注射,同时以等量双蒸水灌胃,联合组以XCFZ中剂量灌胃和顺铂腹腔注射联合给药,模型组按照等量双蒸水灌胃,每天1次,连续给药14 d;称取小鼠瘤质量及去瘤后体质量并计算抑瘤率,Real-time PCR法检测肺癌组织三磷酸腺苷合酶β亚基(ATP 5B)mRNA表达及细胞色素C氧化酶亚单IV(COX IV)mRNA表达水平;高效液相色谱法(HPLC)检测一磷酸腺苷(AMP)、二磷酸腺苷(ADP)、三磷酸腺苷(ATP)含量。TUNEL染色观察肿瘤细胞凋亡。Western blot检测肿瘤组织细胞程序性死亡配体-2(PD-L2)和反义导向分子(RGMb)表达水平。结果:与模型组比较,联合组抑瘤率最高,其次为顺铂组,XCFZ中剂量组。与模型组比较,XCFZ中剂量组去瘤后体质量升高(P<0.05),顺铂组降低(P<0.05)。与模型组比较,顺铂组及XCFZ高、中剂量组ATP 5B mRNA,COX IV mRNA表达显著降低(P<0.05)。与模型组比较,各给药组ATP含量均降低(P<0.05或P<0.01);与顺铂组比较,联合组ATP含量显著降低(P<0.05或P<0.01);与模型组比较,联合组及XCFZ各剂量组ADP/ATP及AMP/ATP显著升高(P<0.05或P<0.01);与顺铂组比较,XCFZ中剂量组ADP/ATP及AMP/ATP显著升高(P<0.05或P<0.01)。TUNEL染色可见模型组肿瘤细胞凋亡稀少,而各给药组肿瘤细胞凋亡明显增多;各给药组肿瘤细胞稀疏,细胞间空洞较多;DDP组和联合组可见大量坏死细胞,肿瘤细胞抑制萎缩,伴大量碎裂和固缩细胞核及多见胞间空洞。与模型组比较,各用药组PD-L2和RGMb蛋白表达水平显著降低(P<0.05);与顺铂组比较,联合组及XCFZ中、高剂量组PD-L2/RObjective:To investigate the anti-tumor effect and mechanism of Xiangcao Fuzheng Mixture(XCFZ)on Lewis lung cancer in mice.Methods:Mice bearing lung cancer were randomly divided into model group(M),positive control group(DDP),low-dose XCFZ group(LD),medium-dose XCFZ group(MD),high-dose XCFZ group(HD),and combination group(MD+DDP),with 10 mice in each group.From the second day of modeling,the XCFZ low,medium and high dose groups were given 9.3,18.6,37.2 g/kg by gavage,respectively.The rats in the Cisplatin group were given intraperitoneal injection of 5 mg/kg Cisplatin solution every other day,and the rats in the combination group were given medium dose of XCFZ and intraperitoneal injection of Cisplatin.The rats in the model group were given the same amount of 0.9%sodium chloride so-lution once a day for 14 days.The tumor weight of mice was weighed and the tumor inhibition rate was calculat-ed.The expression of ATP 5B mRNA and COX IV mRNA in lung cancer tissues was detected by Real-time PCR.The contents of AMP,ADP and ATP were detected by HPLC.The apoptosis of tumor cells observed by TUNEL staining.The expression levels of programmed death ligand-2(PD-L2)and antisense guide molecule(RGMb)in tumor tissues were detected by Western blot.Results:Compared with the model group,the tumor inhibition rate of the combined group was the highest,followed by the Cisplatin group and the medium dose XCFZ group.Compared with the model group,the body weight of the XCFZ medium dose group was increased after tumor removal(P<0.05),and that of the Cisplatin group was decreased(P<0.05).Compared with the model group,the expressions of ATP 5B mRNA and COX IV mRNA in the Cisplatin group and the XCFZ high and medium dose groups were significantly decreased(P<0.05).Compared with the model group,the content of ATP in each medication group was decreased(P<0.05 or P<0.01).Compared with Cisplatin group,ATP content in combination group was significantly decreased(P<0.05 or P<0.01).Compared with the model group,ADP/ATP and AMP/ATP in the combinat

关 键 词：香草扶正合剂 肺癌 ATP 5B COX IV PD-L2/RGMb 小鼠 

分 类 号：R285.5[医药卫生—中药学]