The careful selection of zwitterionic nanoparticle coating results in rapid and efficient cell labeling for imaging-based cell tracking  

作　　者：Nicholas D.Calvert Luciana Yu Olivia C.Sehl Julia J.Gevaert Natasha N.Knier Angelie Rivera-Rodriguez Clara S.Goulet Nitara Fernando Samantha Flood Carlos M.Rinaldi-Ramos Paula J.Foster Adam J.Shuhendler 

机构地区：[1]Department of Chemistry and Biomolecular Sciences,University of Ottawa,Ottawa,Ontario,Canada [2]Department of Medical Biophysics,Western University,London,Ontario,Canada [3]Imaging Research Laboratories,Robarts Research Institute,London,Ontario,Canada [4]J.Crayton Pruitt Family Department of Biomedical Engineering,University of Florida,Gainesville,Florida,USA [5]Department of Chemical Engineering,University of Florida,Gainesville,Florida,USA [6]Department of Biology,University of Ottawa,Ottawa,Ontario,Canada [7]University of Ottawa Heart Institute,Ottawa,Ontario,Canada

出　　处：《Aggregate》2024年第6期173-186,共14页聚集体(英文)

基　　金：Natural Sciences and Engineering Research Council of Canada,Grant/Award Number:RGPIN-2021-03387;Canadian Institutes of Health Research,Grant/Award Number:PJT-178223。

摘　　要：The increased clinical application of cell-based therapies has resulted in a parallel increase in the need for non-invasive imaging-based approaches for cell tracking,often through labeling with nanoparticles.An ideal nanoparticle for such applications must be biologically compatible as well as readily internalized by cells to ensure adequate and stable cell loading.Surface coatings have been used to make nanoparticle trackers suitable for these purposes,but those currently employed tend to have cytotoxic effects.Zwitterionic ligands are known to be biocompatible and antifouling;however,head-to-head evaluation of specific zwitterionic ligands for cell loading has not yet been explored.Magnetic particle imaging(MPI)detects superparamagnetic iron oxide nanoparticles(SPIONs)using time-varying magnetic fields.BecauseMPI can produce high-contrast,real-time images with no tissue depth limitation,it is an ideal candidate for in vivo cell tracking.In this work,we have conjugated hard(permanently charged)and soft(pKa-dependently charged)biomimetic zwitterionic ligands to SPIONs and characterized how these ligands changed SPION physicochemical properties.We have evaluated cellular uptake and subcellular localization between zwitterions,how the improvement in cell uptake generated stronger MPI signal for smaller numbers of cells,and how these cells can be tracked in an animal model with greater sensitivity for longer periods of time.Our best-performing surface coating afforded high cell loading within 4 h,with full signal retention in vivo over 7 days.

关 键 词：cell tracking magnetic nanoparticle imaging(MPI) nanoparticle functionalization nanoparticle uptake surface chemistry zwitterionic ligands 

分 类 号：TG1[金属学及工艺—金属学]