基于UPLC-QTOF-MS/MS技术的3种基原重楼化学成分鉴定及抗肿瘤和抗炎活性研究  

The chemical composition identification based on UPLC-QTOF-MS/MS technology and anti-tumor,anti-inflammatory activity studies of 3 species of Paris

作　　者：彭锐 纪亮 谢天择 刘煜飞 陈两绵[2] 刘涛[3] 王智民[2] 高慧敏[2] Peng Rui;Ji Liang;Xie Tianze;Liu Yufei;Chen Liangmian;Liu Tao;Wang Zhimin;Gao Huimin(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046;Institute of Chinese Materia Medica,National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,China Academy of Chinese Medical Sciences,Beijing 100700;School of Food and Bioengineering,Chengdu University,Chengdu 610106)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]中国中医科学院中药研究所,中药质量控制技术国家工程实验室,北京100700 [3]成都大学食品与生物工程学院,成都610016

出　　处：《中国抗生素杂志》2024年第12期1399-1410,共12页Chinese Journal of Antibiotics

基　　金：中国中医科学院中药研究所“使命导向改革”重点专项(No.CI2023E001TS03);国家自然科学基金(No.82173996);国家重点研发计划项目(No.2023YFC3504000)。

摘　　要：目的明确北重楼与法定基原重楼滇重楼和华重楼的化学差异性及其抗肿瘤和抗炎活性差异,为北重楼资源的合理利用和开发提供参考。方法利用UPLC-QTOF-MS/MS技术对3种基原重楼的化学成分进行表征,并探索是否含吡咯里西啶生物碱类成分;采用对肝癌HepG2细胞、肺癌A549细胞增殖的抑制作用评价抗肿瘤活性;采用LPS诱导的RAW264.7细胞释放NO模型评价抗炎活性。结果从3种基原重楼中鉴定出67个化合物,其中53个为甾体皂苷,在北重楼中鉴定到22个甾体皂苷,未鉴定到吡咯里西啶生物碱。在试验药物浓度下,北重楼仅对A549细胞增殖有抑制作用,IC50为141.0μg/mL,而3种基原重楼在无毒浓度下对LPS诱导RAW264.7细胞释放NO的抑制作用均不显著。结论与法定基原重楼相比,北重楼含有的甾体皂苷成分种类较少,且组成上以多羟基类甾体皂苷为主;从本实验北重楼样本中未发现已分离得到的吡咯里西啶生物碱。在药理活性方面,北重楼具有潜在的抗肺癌活性。本研究为进一步明确北重楼的药效物质以及其资源的开发利用提供初步参考。Objective To elucidate the bariations in the chemical,anti-tumor,and anti-inflammatory activitity of Paris polyphylla var.yunnanensis(PPY),Paris polyphylla var.chinensis(PPC)and Paris verticillata,and to serve as a guide for the rational utilization and advancement of Paris verticillata resources.Methods UPLC-QTOF-MS/MS was utilized to characterize the chemical constituents of 3 species of Paris to explore whether it contained pyrrolizidine alkaloids;the anti-tumor activity was evaluated by inhibiting the proliferation of HepG2 cells and A549 cells;and the anti-inflammatory activity was evaluated by the LPS-induced NO release model in RAW264.7 cells.Results Sixty-seven compounds were identified from the 3 species of Paris,53 of which were steroidal saponins.A total of 22 steroidal saponins were identified from Paris verticillate,and pyrrolizidine alkaloids were not identified.At the test drug concentration,Paris verticillate only inhibited the proliferation of A549 cells with an IC50 of 141.0μg/mL,while the 3 species of Paris had no significant inhibitory effect on the release of NO from LPS-induced RAW264.7 cells at non-toxic concentrations.Conclusion Compared to PPY and PPC,Paris verticillate contained fewer types of steroidal saponins,primarily composed of polyhydroxysteroid saponins;the isolated pyrrolizidine alkaloids were not found in the samples of Paris verticillate in this experiment.In terms of pharmacological activity,Paris verticillate had potential anti-lung cancer activity.This study provided a preliminary reference for further clarifying the effective substances of Paris verticillata and the development and utilization of its resources.

关 键 词：北重楼 甾体皂苷 吡咯里西啶生物碱 化学组成 抗肿瘤 抗炎 

分 类 号：R9[医药卫生—药学]