基于UHPLC-Q-Orbitrap HRMS技术探究芪葛汤在高脂血症大鼠中的入血成分及其代谢产物  

Ingredients Absorbed into Blood and Their Metabolites of Qige Decoction in Hyperlipidemia Rats Based on UHPLC-Q-Orbitrap HRMS

作　　者：李燕芳 郭凯欣 陈为[2] 叶晋通 凡思敏 唐慧[1] 黄可儿[2] 柯雪红[2] LI Yanfang;GUO Kaixin;CHEN Wei;YE Jintong;FAN Simin;TANG Hui;HUANG Ke’er;KE Xuehong(Guangzhou University of Chinese Medicine,Guangzhou 510405,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中国现代应用药学》2024年第22期3061-3070,共10页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(82074099);广州中医药大学“双一流”与高水平大学学科协同创新团队项目(2021xk33);广东省基础与应用基础研究基金项目(2023A1515011126)。

摘　　要：目的探究和表征芪葛汤在高脂血症大鼠中的入血成分,为其药效物质基础研究提供参考。方法以高脂饲料喂养建立高脂血症大鼠模型,灌胃给予芪葛汤并收集血清样品,利用UHPLC-Q-Orbitrap HRMS技术分析鉴定吸收入血的原形成分及其代谢产物。结果共鉴定出46个化学成分,其中15个为原型成分,31个为代谢产物。15个原型成分中7个来自黄芪,5个来自葛根,3个来自广陈皮;31个代谢产物主要是来自含有毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、染料木素、葛根素、大豆苷元、川陈皮素、橙皮素等结构单元的化合物代谢物。其主要代谢途径为羟基化、还原反应、氧化反应、水解反应、去甲基反应、糖醛酸化及其复合反应、葡萄糖醛酸化及其复合反应。结论毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、染料木素、葛根素、大豆苷元、川陈皮素、橙皮素在大鼠体内主要代谢途径为Ⅰ相和Ⅱ相代谢。该方法能快速分析芪葛汤的入血成分及其代谢产物。OBJECTIVE To profile and characterize the ingredients absorbed into blood and their metabolites of Qige decoction in hyperlipidemia rats,and so as to provide a reference for investigation of the pharmacodynamicsubstances of Qige decoction.METHODS Rats model with hyperlipidemia induced by high-fat diet feeding were intragastrically administered with Qige decoction,and the rat plasma samples were collected and analyzed by UHPLC-Q-Orbitrap HRMS to identify the prototype ingredients absorbed into blood and their metabolites.RESULTS A total of 46 chemical components were identified,of which 15 were prototype components and 31 were metabolites.Among the 15 prototype components,7 were from Astragali Radix,5 were from Puerariae Lobatae Radix,3 were from Citri Reticulatae Chachiensis Pericarpium,the 31 metabolites were mainly derived from complex metabolites,which contained structural units including calycosin,calycosin 7-O-glucoside,genistein,puerarin,daidzein,nobiletin and hesperetin.The main metabolic pathways were hydroxylation,reduction reaction,oxidation reaction,hydrolysis reaction,demethylation reaction,uronidation and its complex reaction,glucuronidation and its complex reaction.CONCLUSION The main metabolic pathways of calycosin,calycosin 7-O-glucoside,genistein,puerarin,daidzein,nobiletin and hesperetin in rats are phaseⅠand phaseⅡmetabolism.This method can quickly analyze the ingredients absorbed into blood and their metabolites of Qige Decoction.

关 键 词：UHPLC-Q-Orbitrap HRMS 芪葛汤 高脂血症 入血成分 代谢产物 

分 类 号：R284.1[医药卫生—中药学] R971.101[医药卫生—中医学]