香芹酚辅助庆大霉素杀灭铜绿假单胞菌效果分析  

Analysis on the Effect of Carvacrol-assisted Gentamicin on Killing Pseudomonas aeruginosa

作　　者：王玉婵 陈稼骏 陈丹妍 刘俊辉 王妍 WANG Yu-chan;CHEN Jia-jun;CHEN Dan-yan;LIU Jun-hui;WANG Yan(Fujian Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation,College of Life Science,Fujian Normal University,Fuzhou,Fujian 350108,China)

机构地区：[1]细胞逆境响应与代谢调控福建省高校重点实验室/福建师范大学生命科学学院,福建福州350108

出　　处：《福建农业科技》2024年第12期53-59,共7页Fujian Agricultural Science and Technology

基　　金：福建省自然科学基金青年创新项目(2018J05056)。

摘　　要：铜绿假单胞菌是一种能引起禽类、畜类多种疾病的多重耐药菌,其外膜通透性远低于其他杆菌科细菌,是造成其耐药性和临床治疗失败的重要原因。通过测试不同浓度的香芹酚与庆大霉素在不同处理条件(包括浓度、时间及介质)下,对铜绿假单胞菌的杀伤作用,并评估对铜绿假单胞菌细胞膜破坏的程度,进一步探究香芹酚作为佐剂辅助庆大霉素对铜绿假单胞菌PAO1的杀伤作用。结果表明:在0.01%香芹酚的辅助下,庆大霉素的用量可降低百倍,在1 h内即可显著增强对铜绿假单胞菌的杀菌效果。辅助效果于不同介质中具有适应性,在不同抗生素下也具有一定的广谱性,并且能够提高庆大霉素对临床铜绿假单胞菌杀伤效果。研究结果为香芹酚作为抗菌佐剂在降低铜绿假单胞菌耐药性方面的应用提供了有力的数据支持。Pseudomonas aeruginosa is a multidrug-resistant bacterium that can cause a variety of diseases in poultry and livestock,and its outer membrane permeability is much lower than that of other bacillaceae,which is an important reason for its drug resistance and clinical treatment failure.By testing the killing effect of different concentrations of carvacrol and gentamicin on Pseudomonas aeruginosa under different treatment conditions(including concentration,time and medium),the degree of damage to the cell membrane of Pseudomonas aeruginosa was evaluated,and the killing effect of carvacrol as an adjuvant on gentamicin against Pseudomonas aeruginosa PAO1 was further explored.The results showed that with the assistance of 0.01%carvacrol,the dosage of gentamicin could be reduced by 100 times,and the bactericidal effect on Pseudomonas aeruginosa could be significantly enhanced within 1 hour.The auxiliary effect was adaptable to different media,and also had a certain broad-spectrum activity under different antibiotics,and could improve the killing effect of gentamicin on the clinical Pseudomonas aeruginosa.The results of this study provided strong data support for the application of carvacrol as an antimicrobial adjuvant in reducing the drug resistance of Pseudomonas aeruginosa.

关 键 词：细菌耐药 铜绿假单胞菌 佐剂 香芹酚 辅助 

分 类 号：S855.1[农业科学—临床兽医学]