Enhanced long-lasting luminescence nanorods for ultrasensitive detection of SARS-CoV-2 N protein  

用于SARS-CoV-2 N蛋白超灵敏检测的增强型长余辉发光纳米棒

作　　者：Yi Wei Menglin Song Lihua Li Yingjin Ma Xinyue Lao Yuan Liu Guogang Li Jianhua Hao 魏忆;宋梦麟;李丽华;马颖瑾;老欣悦;刘源;李国岗;郝建华(Department of Applied Physics,The Hong Kong Polytechnic University,Kowloon 999077,Hong Kong,China;Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China;Department of Gastrointestinal Surgery,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]Department of Applied Physics,The Hong Kong Polytechnic University,Kowloon 999077,Hong Kong,China [2]Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430074,China [3]Department of Gastrointestinal Surgery,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China

出　　处：《Science China Materials》2025年第1期253-260,共8页中国科学(材料科学)(英文版)

基　　金：supported by a grant from the Research Grants Council of Hong Kong (CRF No. PolyU C5110-20G);Poly U Grants (G-SB4G, 1-CD4S and 1-CE0H)。

摘　　要：Persistent luminescence nanomaterials can remain luminescence when the light source is turned off,which exhibits promise in biosensor and bioimaging fields since they have the ability to completely eradicate tissue autofluorescence.Although significant progress has been made in the persistent luminescence biosensing,there is still a dearth of long-afterglow detection platform with low limit of detection(LOD)and high sensitivity.Herein,Zn_(2)GeO_(4):Mn,Cr persistently luminescent nanorods(PLNRs)with superior persistent luminescence and long afterglow time were developed.The addition of Cr^(3+)manifestly improves persistent luminescence intensity and afterglow duration through creating a deep defect trap.Then the biosensors were constructed by combining the Zn_(2)GeO_(4):Mn,Cr PLNRs-antibody and Fe_(3)O_(4) magnetic nanoparticles(MNPs)-antibody for nucleocapsid protein detection based on electrostatic attraction.The LOD value for nucleocapsid protein realizes as low as 39.82 ag/mL,which is much lower than the previously reported persistent luminescent-based biosensors.Accordingly,the low detection sensitivity is attributed to fluorescence resonance energy transfer.In addition,high specificity is also achieved.Therefore,the as-prepared Zn_(2)GeO_(4):Mn,Cr persistently luminescent materials can act as the promising candidate in biosensors applications.This strategy provides effective guidance for the development of biosensing platforms with high sensitivity and specificity.长余辉发光纳米材料关闭光源仍能保持发光,由于其能够完全消除自发组织荧光,在生物传感和生物成像领域展示了潜在应用前景.尽管长余辉发光材料已经取得了较大的进展,但仍缺少具有低检测限和高灵敏度的长余辉检测平台.基于此,本文开发了具有优异长余辉和超长余辉时间的Zn_(2)GeO_(4):Mn,Cr纳米棒.通过创建深缺陷, Cr^(3+)的共掺杂明显提高了余辉强度和余辉时间.将Zn_(2)GeO_(4):Mn,Cr-抗体和 Fe_(3)O_(4)磁性纳米粒子-抗体构建成生物传感器,用于N蛋白检测.获得的检测限可低至39.82 ag/mL,远低于之前报道的长余辉发光生物传感器,这归因于荧光共振能量传递.此外,构建的长余辉生物传感器还实现了高检测特异性.因此,所制备的Zn_(2)GeO_(4):Mn,Cr长余辉发光材料可作为生物传感器的候选材料.该策略为开发具有高灵敏度和特异性的生物传感平台提供了有效的指导.

关 键 词：Zn_(2)GeO_(4):Mn Cr persistent luminescence BIOSENSOR nucleocapsid protein high sensitivity 

分 类 号：TB383.1[一般工业技术—材料科学与工程] O657.3[理学—分析化学] TP212.3[理学—化学]