miR164家族及其调控种子发育与种子活力的研究进展  

Advances on the miR164 Family and Its Roles in Regulating Seed Development and Vigor

作　　者：姜孝成[1] 田建红 黄科瑞[1,2] 周诗琪 陈婷 唐倪文 JIANG Xiaocheng;TIAN Jianhong;HUANG Kerui;ZHOU Shiqi;CHEN Ting;TANG Niwen(College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China;College of Life and Environmental Science,Hunan University of Arts and Science,Changde 415000,Hunan,China;Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,Jiangxi,China)

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081 [2]湖南文理学院生命与环境科学学院,湖南常德415000 [3]江西省农业科学院水稻研究所,江西南昌330200

出　　处：《生命科学研究》2024年第6期471-482,502,共13页Life Science Research

基　　金：国家自然科学基金资助项目(32072125);湖南省发育生物学工程与新产品协同创新中心项目(20134486)。

摘　　要：微RNA(microRNA,miRNA)是一类20~24 nt的内源性单链非编码小RNA,通过序列互补靶基因的m RNA,在转录后水平沉默或下调其表达。miR164是植物中特有的一个miRNA家族。最新发布的miRBase数据库22.1版收录了涉及40个物种的129个miR164家族成员,其中84个成员的序列均为5′-UGGAGAAGCAGGGCACGUGCA-3′,其他成员的序列与之相比存在1~5 nt的差异。本文主要综述了植物miRNA的形成及作用机制、miR164家族成员的序列差异及其调控种子发育与种子活力的研究进展,以期为通过基因工程操纵miRNA的表达变化来提高农作物种子活力或抗老化能力的分子育种技术提供新思路。MicroRNAs(miRNAs)are a class of 20~24 nt long endogenous single-stranded non-coding small RNAs.They silence or down-regulation of the expression of target genes through sequentially complementing their mRNAs at the post-transcription level.miR164 is a miRNA family unique to plants.The newly released miRBase database 22.1 version contains 129 members of the miR164 family from 40 species,84 of which are 5忆-UGGAGAAGCAGGGCACGUGCA-3忆.The sequences of other members are different by 1~5 nt.Herein,the formation and mechanism of miRNAs in plants,sequence differences among miR164 family members and their regulatory roles in seed development and vigor were reviewed,in order to provide new ideas for molecular breeding to improve seed vigor or anti-aging ability of crops through miRNA expression control by genetic engineering.

关 键 词：微RNA(miRNA) miR164 靶基因 种子发育 种子活力 

分 类 号：Q943.2[生物学—植物学]