禽白血病病毒抗体间接ELISA检测方法的建立与应用  

Establishment and Application of an Indirect ELISA Method for Detecting Antibodies against Avian Leukemia Virus

作　　者：苏晓月[1] 吕转平[1] 沈亚安 赵明明 唐思静[1] SU Xiaoyue;L Zhuanping;SHEN Ya'an;ZHAO Mingming;TANG Sijing(Xinjiang Bayingolin Vocational and Technical College,Korla,Xinjiang 841000,China;Bazhou Food and Drug Inspection Institute,Korla,Xinjiang 841000,China)

机构地区：[1]新疆巴音郭楞职业技术学院,新疆库尔勒841000 [2]巴州食品药品检验所,新疆库尔勒841000

出　　处：《家畜生态学报》2025年第1期64-69,共6页Journal of Domestic Animal Ecology

基　　金：2022年科技特派员农村科技创业行动计划项目(2022KF044);巴音郭楞职业技术学院2024年度院级课题(bykj2024zk-5)。

摘　　要：为了建立一种禽白血病病毒(ALV)抗体检测方法,利用原核表达禽白血病病毒的p27蛋白建立一种检测ALV抗体的间接ELISA方法。结果显示,p27蛋白以包涵体形式表达,Western blot检测显示p27蛋白具有良好的反应活性,以p27蛋白为包被抗原建立的间接ELISA方法检测NDV、IBDV、IBV、AIV-H9、ILTV阳性血清均为阴性,最低抗体检出效价为1︰12800,与进口试剂盒的符合率为94.87%,检测临床血清样品的阳性率为14.02%。结果说明该研究建立的间接ELISA方法特异性强、敏感性高、符合性好,可为ALV的检测和净化等提供一种简单快速的方法。To establish a method for detecting antibodies against avian leukemia virus(ALV),this study established an indirect ELISA method for detecting ALV antibodies using prokaryotic expression of p27 protein.The results showed that the p27 protein was expressed in the form of inclusion bodies,and Western blot analysis showed that the p27 protein had good reactivity.The indirect ELISA method established using p27 protein as the coating antigen for detecting NDV,IBDV,IBV,AIV-H9,and ILTV positive serum was negative,with a minimum antibody titer of 1∶12800.The coincidence rate with the imported kit was 94.87%,and the positive rate for detecting clinical serum samples was 14.02%.In summary,the indirect ELISA method established in this study has strong specificity,high sensitivity,and good consistency,and will provide a simple and fast method for the detection and purification of ALV.

关 键 词：禽白血病病毒 间接ELISA方法 抗体检测 净化 

分 类 号：S852.65[农业科学—基础兽医学]