芳基硼酸类荧光探针及汞离子高灵敏选择性检测  

Aryl boronic acid-based fluorescent probes for mercury ions detection with high sensitivity and selectivity

作　　者：杨翠文 蔡萌 彭桑珊 汪秋安[1] 张俊丰 刘承斌[1,2] 何清 YANG Cuiwen;CAI Meng;PENG Sangshan;WANG Qiuang;ZHANG Junfeng;LIU Chengbin;HE Qing(College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China;College of Environmental and Resource Science,Xiangtan University,Xiangtan 411105,China)

机构地区：[1]湖南大学化学化工学院,湖南长沙410082 [2]湘潭大学环境与资源学院,湖南湘潭411105

出　　处：《有色设备》2024年第6期29-35,共7页Nonferrous Metallurgical Equipment

基　　金：国家重点研发计划项目(2020YFC1807301)。

摘　　要：针对现有芳基硼酸荧光探针响应时间较慢、灵敏度偏低、激发波长偏短等问题,本研究通过从头设计合成的方法,基于“三合一”策略对芳基硼酸荧光探针中芳基部分的π共轭体系进行了扩展。结果表明,新发展的荧光探针在10 mM HEPES缓冲液中最大激发波长可达365 nm,最大发射波长为420 nm;其对二价汞离子的检测限低至0.6 ppb,响应时间小于1 min,对甲基汞的检测限为2.8 ppb,响应时间为小于8 min。更重要的是,在实际水样如自来水、河水和地下水检测中,荧光探针对二价汞和甲基汞离子的检测限依然可低到5 ppb。该研究为发展超高灵敏、超高选择性的新一低汞/甲基汞离子荧光探针提供了新思路。To address the limitations of existing arylboronic acid-based fluorescent probes,such as relatively slow response time,low sensitivity,and short excitation wavelengths,this study presents a de novo design and synthetic approach.Utilizing a“three-in-one”strategy,we expanded theπ-conjugated system of the aryl group in the fluorescent probes.The newly developed probe exhibits a maximum excitation wavelength of 365 nm and a maximum emission wavelength of 420 nm in 10 mM HEPES buffer.It achieves an impressive detection limit of 0.6 ppb for divalent mercury(Hg^(^(2+)))with a response time of less than 1 minute,and a detection limit of 2.8 ppb for methylmercury(CH_(3)Hg^(+))with a response time of under 8 minutes.Importantly,the probe maintains excellent performance in real water samples,including tap water,river water,and groundwater,achieving detection limits as low as 5 ppb for Hg^(^(2+))and CH_(3)Hg^(+).This work provides a promising strategy for developing highly sensitive and selective fluorescent probes for the detection of mercury and methylmercury ions.

关 键 词：荧光探针 芳基硼酸 汞离子 甲基汞 地下水 

分 类 号：X824[环境科学与工程—环境工程]