湖北贝母内生真菌Fusarium foetens化学成分研究  

Chemical constituent and anti-inflammatory activity of endophytic Fungus Fusarium foetens from Fritillaria hupehensis

作　　者：同舜尧 刘晴 何瑜 徐帆 石宝宝 艾洪莲[1] TONG Shunyao;LIU Qing;HE Yu;XU Fan;SHI Baobao;AI Honglian(School of Pharmaceutical Sciences,South-Central Minzu University,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报(自然科学版)》2025年第2期183-187,共5页Journal of South-Central Minzu University(Natural Science Edition)

基　　金：湖北省自然科学基金资助项目(2022CFB462);植物化学与西部植物资源持续利用国家重点实验室开放基金资助项目(P2022-KF03)。

摘　　要：采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、MPLC和(半)制备HPLC的方法对湖北贝母内生真菌Fusarium foetens固体发酵产物的乙酸乙酯萃取物进行研究,共分离得到10个化合物,通过波谱学数据分析并结合其物理性质,将其鉴定为:n-苯乙基乙酰胺(1)、fusaric acid(2)、beauvericin(3)、methylp-hydroxy benzeneacetate(4)、fumitremorginx(5)、1-acetyl-2,4,5,7-tetrahydroxy-9(6)、5,5'-dimethoxybiphenyl-2,2'-diol(7)、p-(acetylamino)benzoic acid(8)、gibepyrone D (9)、5-羟甲基-2-呋喃甲醛(10).其中化合物5为新化合物.采用脂多糖(LPS)诱导的RAW 264.7巨噬细胞产生NO炎症效应对以上化合物进行抗炎活性检测,其中化合物8表现出良好的抗炎活性(IC50=22.91μM).By using gel column chromatography,Sephadex LH-20,MPLC,and(semi-)preparative HPLC,the chemical constituent of Fusarium foetens from Fritillaria hupehensis was studied,from which 10 compounds were obtained.According to the spectroscopic data,combined with their physical and chemical properties,their structures were identified as n-phenethylacetamide(1),fusaric acid(2),beauvericin(3),methylp-hydroxy benzeneacetate(4),fumitremorgin x(5),1-acetyl-2,4,5,7-tetrahydroxy-9(6),5,5'-dimethoxybiphenyl-2,2'-diol(7),p-(acetylamino)benzoic acid(8),gibepyrone D(9),5-hydroxymethylfurfural(10),in which compound 5 wase first reported as a natural product.All compounds were evaluated for anti-inflammatory activity,and compound 8 showed great anti-inflammatory activity.

关 键 词：湖北贝母 内生真菌 化学成分 抗炎活性 

分 类 号：R931[医药卫生—生药学]