Rapid and specific fluorescent probe visualizes dynamic correlation of Cys and HClO in OGD/R  

作　　者：Pei Huang Weijie Zhang Junping Wang Fangjun Huo Caixia Yin 

机构地区：[1]Institute of Molecular Science,Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China [2]Research Institute of Applied Chemistry,Shanxi University,Taiyuan 030006,China

出　　处：《Chinese Chemical Letters》2025年第1期437-441,共5页中国化学快报(英文版)

基　　金：the National Natural Science Foundation of China(Nos.22207069,22325703,22377071,22074084);Research Project Supported by Shanxi Scholarship Council of China(No.2022-002);the Shanxi Province Science Foundation(Nos.20210302124012,202203021221009);Key R&D and transformation plan of Qinghai Province(No.2020-GX-101);2023 Graduate Innovation Project of Shanxi University。

摘　　要：Intracellular redox homeostasis is of indispensable importance in pathophysiology.In order to maintain the balance of the redox state within the cell,reactive oxygen species(ROS)and reactive sulfur species(RSS)react and transform with each other,and their levels also directly reflect the degree of oxidative stress and disease.Hypochlorous acid(HClO)and cysteine(Cys)usually co-exist in organisms,interacting with each other in many important physiological processes and synergistically maintaining the dynamic redox balance in the body.To understand the relevance and pathophysiological effects of these two signaling molecules in oxidative stress,unique fluorescence imaging tools are required.Herein,we designed and developed a dual-channel fluorescent probe HP,for the individual and continuous detection of HClO and Cys.This probe could simultaneously monitor the changes in the concentrations of HClO and Cys in cells,and was characterized by a fast response,high sensitivity and high selectivity,especially compared with glutathione(GSH)and homocysteine(Hcy),the probe had a good specificity for Cys.Importantly,probe HP successfully observed dynamic changes in HCl O-and Cys-mediated redox status in the oxygenglucose deprivation/reperfusion(OGD/R)model of HeLa cells and dynamically monitored fluctuations in endogenous HClO levels in lipopolysaccharides(LPS)-induced peritonitis mice.

关 键 词：Redox homeostasis DUAL-CHANNEL OGD/R FLUCTUATIONS PERITONITIS 

分 类 号：O657.3[理学—分析化学] Q503[理学—化学]