Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples  

作　　者：Miao-Miao Chen Min-Ling Zhang Xiao Song Jun Jiang Xiaoqian Tang Qi Zhang Xiuhua Zhang Peiwu Li 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Laboratory of Risk Assessment for Oilseed Products(Wuhan),Quality Inspection and Test Center for Oilseed Products,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China [2]Food Safety Research Institute,Hubei University,Wuhan 430062,China [3]Hubei Hongshan Laboratory,Wuhan 430070,China [4]Xianghu Laboratory,Hangzhou 311231,China [5]College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,China

出　　处：《Chinese Chemical Letters》2025年第1期464-468,共5页中国化学快报(英文版)

基　　金：financially supported by China Postdoctoral Science Foundation(No.2022T150708);National Key Research and Development Program of China(No.2023YFF1104600);National Natural Science Foundation of China(Nos.32072305,32102089)。

摘　　要：Aflatoxins B1(AFB1)contamination in agro-food holds great threaten to human and animal health.Conventional test strips for rapid AFB1 visualized monitoring remains challenged by improvement of sensitivity and matrix interference resistance.In this case,we developed a portable electrochemiluminescence(ECL)imaging test strip with dual-signal outputs for AFB1 quantification in corn samples.RuPEI@SiO_(2)@Au nanospheres were synthesized for bonding with anti-AFB1 antibody and then colorimetrical signal-reported on test line through the capillary flow at strips.Meanwhile,ECL imaging signal of the constructed carbon-ink-based working electrode on polyvinyl chloride substrate of strips was exported under an applied potential of 1.25 V.The whole ECL test strips not only endowed convenient colorimetric responses but guaranteed quick-witted ECL image distinguishment even at extremely low AFB1 content.The detection limit of this ECL imaging-integrated mode was 10-fold lower than that of only colorimetric mode.Furthermore,satisfactory selectivity,reliability and practicability of the as-proposed ECL test strips were demonstrated.This work offered a promising platform for on-site,accurate and sensitive detection of pollutants in foods.

关 键 词：Electrochemiluminescence imaging Test strips Nanomaterials Dual-signal outputs Aflatoxin B1 Food safety 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.3[轻工技术与工程—食品科学与工程]