Logically integrating exo/endogenous gated DNA trackers for precise micro RNA imaging via synergistic manipulation  

作　　者：Fanghua Zhang Yuyan Li Hongyan Zhang Wendong Liu Zhe Hao Mingzheng Shao Ruizhong Zhang Xiyan Li Libing Zhang 

机构地区：[1]Tianjin Key Laboratory of Molecular Optoelectronic Sciences,Department of Chemistry,Tianjin University,Tianjin 300072,China [2]Institute of Photoelectronic Thin Film Devices and Technology,Solar Energy Conversion Center,Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin,Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education,Nankai University,Tianjin 300350,China

出　　处：《Chinese Chemical Letters》2025年第1期478-483,共6页中国化学快报(英文版)

基　　金：supported by the National Natural Science Foundation of China(Nos.21904095,22004089);Peiyang Talents Project of Tianjin University;Young Thousand Talented Program;Program of Tianjin Science and Technology Major Project and Engineering(No.19ZXYXSY00090)。

摘　　要：Nanomaterials provide an ideal platform for biomolecular display due to their dimensions approach the molecular scale,facilitating binding behavior akin to that observed in solution-based processes.DNA nanoprobes hold great promise as miniature detectives capable of detecting mi RNAs within cells.However,current nanoprobes face a challenge in achieving the required precision for accurate miRNA detection,particularly within the intricate confines of the cellular microenvironment,due to interference with biological autofluorescence,off-target effects,and a lack of spatiotemporal control.Here,we have designed a dual-stimuli responsive DNA tracker,synergistically utilizing specific intracellular cues and external triggers,which enables spatiotemporal-controlled and precise detection and imaging of miRNAs"on demand".The tracker,which combines zeolitic imidazolate framework-67(ZIF-67)and unique hairpin DNA structures,effectively anchored onto the ZIF-67 through electrostatic interactions,remains in a dormant state until activated by abundant cellular ATP,resulting in the release of the hairpin structures that include a PC linker incorporated into the loop region.Subsequent irradiation triggers specific recognition of the target miRNA.The newly developed HP-PC-BT@ZIF-67 tracker demonstrates precise spatiotemporal mi RNA detection and exhibits excellent biocompatibility,enabling specific mi RNA recognition"on demand"within cancer cells.This research presents a reliable mi RNA imaging platform in the intricate cellular environment,opening up the possibilities for precise biomedical analysis and disease diagnosis.

关 键 词：STIMULI-RESPONSIVE DNA tracker Synergistic manipulation Spatiotemporal imaging microRNAs 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R318[医药卫生—生物医学工程]