尖锐湿疣皮损中miR-34a-5p和miR-203表达水平与临床特征及HPV感染类型的相关性研究  

Study on the correlation between miR-34a-5p and miR-203 expression and clinical features and HPV infection types in lesions of condyloma acuminatum

作　　者：宋飞娟 梁爱芳[1] 刘俊姣[1] SONG Feijuan;LIANG Aifang;LIU Junjiao(Puyang People's Hospital,Department of Dermatology and Venereology,Puyang 457000,Henan Province)

机构地区：[1]濮阳市人民医院皮肤性病科,河南濮阳457000

出　　处：《皮肤病与性病》2024年第6期347-351,共5页Dermatology and Venereology

摘　　要：目的分析尖锐湿疣(CA)患者皮损中miR-34a-5p、miR-203表达水平与临床特征及HPV感染型别的关系,为CA靶向治疗研究提供参考。方法选取2021年1月~2023年12月到某院诊治的尖锐湿疣患者50例,设为CA组;同期收集50例健康包皮或外阴组织作为对照组。采用实时荧光定量PCR检测两组组织中miR-34a-5p、miR-203表达水平,比较组间差异;单因素分析CA组皮损中的miR-34a-5p、miR-203表达水平与其临床特征、HPV感染型别之间的关系,并进行Spearman相关性分析;绘制受试者工作特征曲线(ROC)探讨miR-34a-5p、miR-203表达水平对CA患者HPV感染类型的诊断价值。结果CA组miR-34a-5p、miR-203表达水平低于正常组(P<0.05)。CA组中疣体直径≥10 mm和HPV高危型感染者的miR-34a-5p、miR-203表达水平较低(P<0.05),其余不同特征之间的比较差异无统计学意义(P>0.05)。CA组miR-34a-5p、miR-203表达水平与其疣体直径、HPV感染类型呈反比(P<0.05)。绘制ROC曲线发现,miR-34a-5p、miR-203表达水平均可用于诊断CA患者HPV感染类型,其单一及联合诊断ROC曲线下面积(AUC)依次为0.683、0.785、0.804,其中联合诊断AUC高于单一因子(P<0.05)。结论CA患者皮损中miR-34a-5p、miR-203表达水平异常下降,与疣体大小、HPV类型呈负相关关系,可用于临床诊断HPV感染类型。Objective To analyze the relationship between the expression levels of miR-34a-5p and miR-203 in skin lesions of patients with condyloma acuminatum(CA)and the clinical characteristics and the type of human papillomavirus(HPV)infection.Methods A total of 50 patients diagnosed with CA treated in our hospital from January 2021 to December 2023 were selected as the study group;while,50 healthy prepuce or vulva tissues were collected as control group.Real-time fluorescent quantitative PCR was used to detect the expression levels of miR-34a-5p and miR-203 in both groups,and the differences were compared.The relationship between the expression levels of miR-34a-5p and miR-203 in CA lesions and clinical features and HPV infection type was analyzed by single factor analysisand Spearman correlation analysis.Additionally,receiver operating characteristic curve(ROC)was drawn to analyze the diagnostic value of miR-34a-5p and miR-203 expression levels on HPV infection types in CA patients.Results The expression levels of miR-34a-5p and miR-203 in CA group were lower than those in normal group(P<0.05).In CA group,the expression levels of miR-34a-5p and miR-203 were higher in patients with warts≥10 mm in diameter and high-risk HPV infections(P<0.05),and there was no statistical significance among other characteristics(P>0.05).An inverse relationship was found between the expression levels of miR-34a-5p and miR-203 and wart diameter and HPV infection type(P<0.05).The ROC curve showed that the expression levels of miR-34a-5p and miR-203 could be used to diagnose HPV infection types in CA patients,and the area under curve(AUC)of single and combined diagnosis was 0.683,0.785,and 0.804,respectively.The combined diagnosis AUC was higher than that of single factor(P<0.05).Conclusion The expression levels of miR-34a-5p and miR-203 in CA patients'skin lesions were abnormally decreased,which was negatively correlated with wart size and HPV type,and could be used for clinical diagnosis of HPV infection type.

关 键 词：尖锐湿疣 miR-34a-5p miR-203 临床特征 人乳头状瘤病毒 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]