基于UPLC-Q-TOF-MS法和网络药理学探讨草归除湿活血颗粒治疗高尿酸血症的机制  

Mechanism of Caogui Chushi Huoxue granules for hyperuricemia based on UPLC-Q-TOF-MS and network pharmacology

作　　者：古丽达力·赛里克 汪建萍[2,5] 林欣悦 赵翡翠 Gulidali Sailike;WANG Jian-ping;LIN Xin-yue;ZHAO Fei-cui(The Fourth Clinical Medical College of Xinjiang Medical University,Urumqi 830054;Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urmuqi 830000;Xinjiang Uygur Autonomous Region Key Laboratory of Traditional Chinese Medicine Processing Research,Urumqi 830000;Inheritance Studio Program of Famous Traditional Chinese Medicine Experts,Urmuqi 830000;Key Laboratory of Xinjiang Uygur Autonomous Region,Laboratory of Famous Prescription and Science of Formulas,Urumqi 830017)

机构地区：[1]新疆医科大学第四临床医学院,乌鲁木齐830054 [2]新疆医科大学附属中医医院,乌鲁木齐830000 [3]新疆维吾尔自治区中药炮制研究重点实验室,乌鲁木齐830000 [4]新疆维吾尔自治区名老中医药专家传承工作室,乌鲁木齐830000 [5]新疆维吾尔自治区重点实验室,新疆名医名方与特色方剂学实验室,乌鲁木齐830017

出　　处：《中南药学》2025年第1期114-123,共10页Central South Pharmacy

基　　金：新疆维吾尔自治区重大科技专项项目(No.2022A03019);新疆维吾尔自治区科学技术厅,重点实验室开放课题(No.2020D04024)。

摘　　要：目的考察草归除湿活血颗粒剂的提取工艺,并探究其通过降尿酸治疗高尿酸症的机制。方法通过HPLC测定草归除湿活血颗粒水提液中各成分的含量,筛选最佳提取条件;利用UPLC-Q-TOF-MS分析其活性成分,再将得到的活性成分结合高尿酸血症的潜在靶点,进行PPI网络构建及GO、KEGG分析。结果最佳提取工艺为10倍水浸泡0.5 h,加热回流提取2次,每次1 h。UPLC-Q-TOF-MS得221个活性成分,网络药理学分析得到183个潜在交集靶点,富集分析得到PI3K-Akt信号通路、脂质和动脉粥样硬化、HIF-1等信号通路。结论草归除湿活血颗粒可能通过调节PI3K-Akt信号通路、脂质和动脉粥样硬化、HIF-1等信号通路来发挥降尿酸的作用。Objective To determine the extraction process of Caogui Chushi Huoxue granules,and its mechanism in lowering the uric acid for hyperuricemia.Methods The content of the components in the aqueous extract of Caogui Chushi Huoxue granules was determined by HPLC,and the optimal extraction conditions were obtained.The active components were analyzed by UPLC-Q-TOF-MS,and then the obtained active components were combined with hyperuricemia targets to serve as potential targets.PPI network was constructed and GO and KEGG analyses conducted.Results The optimal extraction process included 10-fold water soaked for 0.5 h,heating,and reflux extraction twice,1 h for each.Totally 221 active components were obtained by UPLC-Q-TOF-MS,and 183 potential targets by PPI network.The signaling pathways,such as PI3K-Akt pathway,lipid and atherosclerosis,and HIF-1,were obtained by enrichment analysis.Conclusion Caogui Chushi Huoxue granules may regulate the signaling pathways such as TNF,PI3K-Akt,and cancer to lower the uric acid.

关 键 词：草归除湿活血颗粒 高尿酸血症 制备工艺 UPLC-Q-TOF-MS 网络药理学 

分 类 号：R285[医药卫生—中药学] R284.1[医药卫生—中医学]