防风炒炭前后HPLC指纹图谱和4种色原酮成分含量变化研究  

Changes of HPLC fingerprint and contents of 4 chromones components in Saposhnikovia divaricata before and after charcoal-frying

作　　者：王艾琳 吴周威 谢瑜[1] 匡创富[1] 王青[1] 刘钇志 WANG Ai-lin;WU Zhou-wei;XIE Yu;KUANG Chuang-fu;WANG Qing;LIU Yi-zhi(The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005;Hunan Xinhui Pharmaceutical Co.,Ltd.,Changsha 410203)

机构地区：[1]湖南中医药大学第二附属医院,长沙410005 [2]湖南新汇医药有限公司,长沙410203

出　　处：《中南药学》2025年第1期208-214,共7页Central South Pharmacy

基　　金：湖南省中医药管理局项目(No.D2022137);湖南省中医药管理局重大项目(No.A2022005)。

摘　　要：目的建立防风及防风炭HPLC指纹图谱,考察不同产地防风炒炭前后4种色原酮类成分含量,探究防风炒炭过程中各成分变化规律。方法采用Venusil C18 Plus(4.6 mm×250 mm,5μm),以水-甲醇梯度洗脱,流速1.0 mL·min^(-1),检测波长254 nm,柱温30℃。建立防风及防风炭HPLC指纹图谱,通过统计学方法对防风炒炭前后指纹图谱进行分析,并测定升麻素苷、5-O-甲基维斯阿米醇苷、升麻素和亥茅酚苷的含量。结果不同产地防风及防风炭HPLC指纹图谱存在差异。各产地防风炒炭后升麻素苷及5-O-甲基维斯阿米醇苷含量均降低,平均降幅分别为17.94%,6.15%;其中升麻素降低显著,平均降幅为21.14%;亥茅酚苷含量呈上升趋势,平均增幅为10.65%。结论防风炮制前后指纹图谱存在差异,4种色原酮类成分含量变化,可能与防风炭偏于止血有关。本研究为防风炭中活性物质及质量控制提供参考。Objective To establish the HPLC fingerprints of Saposhnikovia divaricata and Saposhnikovia divaricata charcoal,and determine the content of 4 chromones in Saposhnikovia divaricata charcoal from different origins before and after the charcoal-frying,as well as the changing patterns of various components in the process of charcoal-frying.Methods Venusil C18 Plus(4.6 mm×250 mm,5μm)was used with water-methanol gradient elution at a flow rate of 1.0 mL·min^(-1),a detection wave length of 254 nm,and a column temperature of 30℃.HPLC fingerprints of Saposhnikovia divaricata and Saposhnikovia divaricata charcoal were established,and the fingerprints of Saposhnikovia divaricata before and after the charcoal-frying were analyzed statistically.The content of prim-O-glucosylcimifugin,4'-O-β-D-glucosyl-5-O-methylvisamminol,cimifugin,and sec-O-glucosylhamaudol were determined.Results There were differences in the HPLC fingerprints of Saposhnikovia divaricata and Saposhnikovia divaricata charcoal from different origins.The content of prim-O-glucosylcimifugin and 4'-O-β-D-glucosyl-5-O-methylvisamminol in charcoal-fried Saposhnikovia divaricata from various origins decreased by 17.94%and 6.15%on average.Cimifugin decreased most,with an average decrease of 21.14%.The content of sec-O-glucosylhamaudol increased,with an average growth of 10.65%.Conclusion There are differences in the fingerprints of Saposhnikovia divaricata charcoal before and after the processing.The content of 4 chromones varies,which may be related to the hemostatic effect of Saposhnikovia divaricata charcoal.This study provides a reference for the study of active substances and quality control of Saposhnikovia divaricata charcoal.

关 键 词：防风炭 炮制 色原酮 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]