山东中华樱桃资源的ISSR分析  

ISSR analysis of Chinese cherry germplasm resources in Shandong Province

作　　者：曾沛源 谭钺[1] 徐丽[1] 朱敏 魏海蓉[1] ZENG Peiyuan;TAN Yue;XU Li;ZHU Min;WEI Hairong(Shandong Institute of Pomology,Tai'an,Shandong 271000,China)

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《落叶果树》2024年第6期36-41,共6页Deciduous Fruits

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2024A07);国家桃产业技术体系樱桃种质资源鉴定与新种质创制岗位(CARS-30-1-08);中央引导地方科技发展专项资金项目(YDZX2022134)。

摘　　要：以46份中华樱桃种质为试材,进行ISSR引物扩增,根据扩增结果计算遗传距离并进行聚类分析。结果表明,筛选出12条多态性良好的ISSR引物,共获得106个条带,其中多态性条带78个,多态率为73.58%。46份材料间遗传相似系数为0.59~0.94,有效等位基因数、基因多样性、香农信息指数分别为1.36、0.21、0.32,说明山东地区中华樱桃遗传多样性较高。通过UPGMA方法进行聚类分析,12条ISSR引物能够完全区分46份种质,并将其分为7个类群。研究结果为山东地区樱桃种质资源的保存与利用提供参考。In this study,46 Chinese cherry(Prunus pseudocerasus Lindl.)germplasm were used as research materials,and DNA was extracted for ISSR primer amplification,and genetic distances were calculated based on the amplification results and analyzed by clustering.The results showed that 106 bands were obtained by PCR amplification with 12 selected ISSR primers,of which 78 bands were polymorphic,with a polymorphism rate of 73.58%.The genetic similarity coefficients among 46 materials were 0.59~0.94,and the effective allele number,gene diversity.and Shannon's information index were 1.36,0.21,and 0.32,respectively,which indicated that the genetic diversity of Chinese cherry was high in Shandong Province.Cluster analysis by UPGMA method showed that 12 ISSR primers were able to completely distinguish 46 germplasm and classify them into seven taxa.The conclusion showed that the genetic analysis of ISSR molecular markers can provide reference for the conservation and utilization of cherry germplasm resources in Shandong Province.

关 键 词：中华樱桃 ISSR 种质资源 遗传多样性 

分 类 号：S662.5[农业科学—果树学]