葡萄GRF/GIF家族基因对遗传转化再生效率的影响  

Studies on the Efficiency of GRFs/GIFs for Genetic Transformation and Regeneration in Grapevine

作　　者：李敏 李思雨 施紫涵 陈爽 徐炎[1] 刘国甜 LI Min;LI Siyu;SHI Zihan;CHEN Shuang;XU Yan;LIU Guotian(Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture and Rural Affairs,College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学园艺学院,农业农村部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100

出　　处：《园艺学报》2025年第1期51-65,共15页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31972374,32372660,31601716)。

摘　　要：为研究葡萄GRF/GIF家族基因对遗传转化再生效率的影响,对在葡萄基因组中鉴定到10个VvGRF和4个VvGIF基因进行了研究。VvGRF含有家族保守结构域WRC和QLQ,VvGIF含有保守结构域SNH和QG。VvGRF和VvGIF过表达烟草培养1个月后再生情况明显优于空载对照。烟草中过表达VvGRF8-GIF2和VvGRF4-GIF2,再生效率高达73.58%和65.05%,是对照的2.4~2.7倍;过表达rVvGRF8-GIF2(miR396结合的靶位点突变后的VvGRF8-GIF2),再生效率提升效果最好,达到了83.43%,是对照的3.1倍;相对于VvGRF8-GIF2嵌合体的过表达转化,构建的VvGRF8、VvGIF2同源嵌合体VvGRF4-GIF2、VvGRF5-GIF2和VvGRF8-GIF3过表达后的促进再生效果相对较弱。综合试验结果,VvGRF8和VvGIF2有利于提升植物遗传转化的再生效率。In order to study the effect of GRF/GIF family genes on the efficiency of genetic transformation and regeneration,10 VvGRF and 4 VvGIF genes were identified in grape genome.VvGRF contains family conserved domains WRC and QLQ,VvGIF contains conserved domains SNH and QG.The regeneration of tobacco overexpressing VvGRF and VvGIF after one month of cultivation was significantly better than that the empty control.VvGRF8-GIF2 and VvGRF4-GIF2 are overexpressed in tobacco,and the regeneration efficiency is as high as 73.58%and 65.05%,which is 2.4-2.7 times that of control.Overexpression of rVvGRF8-GIF2(VvGRF8-GIF2 after mutation of miR396 binding target site)showed the best regeneration efficiency,reaching 83.43%,which was 3.1 times that of control.Compared with the overexpression transformation of VvGRF8-GIF2 chimera,the promotion effect of constructed VvGRF8,VvGIF2 homologous chimera VvGRF4-GIF2,VvGRF5-GIF2 and VvGRF8-GIF3 after overexpression is relatively weak.According to the experimental results,VvGRF8 and VvGIF2 can improve the regeneration efficiency of plant genetic transformation.

关 键 词：葡萄 GRF GIF 遗传转化 再生 

分 类 号：S663.1[农业科学—果树学]