利用宏基因组测序分析华南地区香蕉线条病毒多样性  

Analysis of the Diversity of Banana Streak Virus Infecting Bananas in South China Using Metagenomic Sequencing

作　　者：陈华宙 刘润沛 卢咏思 饶雪琴[1] CHEN Huazhou;LIU Runpei;LU Yongsi;RAO Xueqin(College of Plant Protection,South China Agricultural University,Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Guangzhou 510642,China)

机构地区：[1]华南农业大学植物保护学院,广东省微生物信号与病害防治重点实验室,广州510642

出　　处：《园艺学报》2025年第1期66-72,共7页Acta Horticulturae Sinica

基　　金：现代农业产业技术体系专项资助(CARS-31)。

摘　　要：通过宏基因组测序对为害中国华南地区香蕉的病毒种类进行分析,发现16种病毒,大多数属于花椰菜病毒科(Caulimoviridae)杆状DNA病毒属(Badnavirus),其中7种为香蕉线条病毒(banana streak virus,BSV)。利用免疫捕捉PCR(immunocaptured PCR,IC-PCR)对宏基因组测序结果进行验证,仅检测到5种BSV,分别是香蕉线条病毒OL(banana streak OL virus,BSOLV)、香蕉线条病毒GF(banana streak GF virus,BSGFV)、香蕉线条病毒MY(banana streak MY virus,BSMYV)、香蕉线条病毒IM(banana streak IM virus,BSIMV)和香蕉线条病毒VN(banana streak VN virus,BSVNV)。利用IC-PCR扩增的这些病毒核苷酸序列与GenBank中相应病毒的一致性高于93%。系统发育进化树分析结果表明,这些病毒属于Clade I,是游离病毒。利用IC-PCR对41个广东香蕉样品进行检测,检测到BSOLV、BSGFV、BSMYV、BSIMV和BSVNV,其中BSOLV为优势BSV。Macrogenomic sequencing was used to analyze virus infecting bananas in South China,and 16 different viruses were found,most of them belonging to the genus Badnavirus of the family Caulimoviridae.Among these,seven were banana streak virus(BSV).To confirm the findings of macrogenomic sequencing,immunocaptured PCR(IC-PCR)was used.The results indicated that five BSVs were identified,namely banana streak OL virus(BSOLV),banana streak GF virus(BSGFV),banana streak MY virus(BSMYV),banana streak IM virus(BSIMV),and banana streak VN virus(BSVNV).The nucleotide sequences of these viruses amplified by IC-PCR showed greater than 93%identity with the corresponding viral sequences in GenBank.Phylogenetic tree analysis revealed that these BSVs belonged to Clade I and were episomal.Additionally,BSOLV,BSGFV,BSMYV,BSIMV,and BSVNV were detected in 41 banana samples from Guangdong by IC-PCR,and BSOLV was identified as the dominant BSV.

关 键 词：香蕉 杆状DNA病毒属 香蕉线条病毒 宏基因组测序 

分 类 号：S668.1[农业科学—果树学]