SlBON1调控番茄植株营养生长机制的研究  

Mechanistic Studies on SlBON1-Regulated Plant Vegetative Growth in Tomato

作　　者：李芮 王稳 杜明辉 刘根忠 马方放 包志龙 LI Rui;WANG Wen;DU Minghui;LIU Genzhong;MA Fangfang;BAO Zhilong(State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《园艺学报》2025年第1期73-87,共15页Acta Horticulturae Sinica

基　　金：山东省泰山学者青年专家项目(tsqn201812034)。

摘　　要：BONZAI1(BON1)是高度保守的钙依赖膜结合蛋白Copine家族成员,具有调控生长发育的重要作用。番茄SlBON1与拟南芥BON1的蛋白序列相似度为66.84%。通过CRISPR-Cas9技术获得两个番茄SlBON1敲除突变体cr6和cr8,分别为61个碱基缺失突变和1个碱基插入突变。与野生型相比,SlBON1敲除突变体的地上和地下部生长均受到显著抑制,下胚轴中核内周期下降,叶片光合速率显著下降,淀粉含量升高。转录组分析显示SlBON1敲除突变体中激素信号转导信号通路、MAPK信号通路以及细胞周期的差异基因富集度较高。推测SlBON1通过调控植物激素代谢、细胞周期及光合作用等促进了番茄植株的营养生长。BONZAI1(BON1)is a conserved calcium-dependent,plasma membrane-binding Copine protein,which is important in regulating plant growth and development.Tomato BON1 homolog(SlBON1)has 66.84%protein sequence similarity to Arabidopsis BON1.Crispr-Cas9 technology was utilized to generate two SlBON1 knockout mutants cr6 and cr8,which have 61-nucleotide missing and one-nucleotide insertion,respectively.The SlBON1 knockout mutants have the stunted growth of both aboveground and underground tissues,decreased endoreduplication in hypocotyls,reduced net photosynthetic rate,and increased starch accumulation in leaves compared to the wild type.Transcriptome analysis reveal that SlBON1 knockout mutants had a number of differentially expressed genes,in which hormone signaling pathway,MAPK signaling pathway and cell cycle regulation pathway are enriched.Taken together,the study suggest that SlBON1 plant hormone metabolism,modulates cell cycle and photosynthesis to promote tomato vegetative growth.

关 键 词：番茄 SlBON1 敲除 营养生长 转录组分析 

分 类 号：S641.2[农业科学—蔬菜学]