茄子SmWRKY4的克隆及耐冷性功能验证  

Cloning and Functional Verification of SmWRKY4 Gene in Cold Tolerance of Solanum melongena

作　　者：杨艳 刘军[2] 周晓慧[2] 刘松瑜 庄勇 YANG Yan;LIU Jun;ZHOU Xiaohui;LIU Songyu;ZHUANG Yong(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,南京210014

出　　处：《园艺学报》2025年第1期101-110,共10页Acta Horticulturae Sinica

基　　金：江苏省自然科学基金项目(BK20221429);江苏省农业科技自主创新项目[CX(23)3026];江苏省重点研发计划项目(BE2021377)。

摘　　要：前期研究发现茄子SmWRKY4的表达受到冷信号抑制。进一步以‘成都墨茄’为材料克隆出SmWRKY4全长序列,分析结果显示其开放阅读框为1518 bp,编码505个氨基酸,含有2个WRKY结构域,属于第Ⅰ类WRKY转录因子。SmWRKY4启动子含有茉莉酸甲酯和水杨酸等激素响应元件及缺氧和低温等逆境胁迫响应元件。系统进化树分析表明,SmWRKY4与番茄和马铃薯的同源蛋白在进化关系上更近。表达模式分析显示SmWRKY4能够响应脱落酸、水杨酸和茉莉酸甲酯等外源植物生长调节剂和冷胁迫,并在茄子茎和花药中高表达。敲除SmWRKY4的突变体能够增强光合作用,降低细胞通透性,提高耐冷性。推测SmWRKY4负调控茄子耐冷性。Previous study showed that the expression level of SmWRKY4 was inhibited by cold stress.The full-length sequence of SmWRKY4 was cloned from‘Chengdu Moqie’in this study.Analysis results revealed that the open reading frame of the SmWRKY4 gene was 1518 bp,encoding 505 amino acids which contained two WRKY domains.Consequently,SmWRKY4 protein belonged to groupⅠWRKY transcription factor.SmWRKY4 promoter contained hormone-response elements including MeJA and SA and stress-response elements including oxygen deficit and low temperature.Phylogenetic tree analysis suggested that SmWRKY4 protein was more closely related to its homologous proteins in tomato and potato.Expression pattern analysis showed that SmWRKY4 could respond to ABA,SA,MeJA and cold stress,and was highly expressed in eggplant stem and anther.SmWRKY4 mutation could enhance photosynthesis,reduce cell permeability and improve cold tolerance.These results suggested that SmWRKY4 negatively regulated cold tolerance in eggplant.

关 键 词：茄子 SmWRKY4 耐冷性 克隆 功能验证 光合作用 细胞通透性 激素 

分 类 号：S641.1[农业科学—蔬菜学]