长豇豆Lhc家族基因鉴定及其在盐胁迫下的表达分析  

Identification of Long Cowpea Lhc Gene Family and Its Expression Analysis Under Salt Stress

作　　者：王珊珊 郭瑞 何棱 吴春红 陈禅友 万何平 赵慧霞 WANG Shanshan;GUO Rui;HE Ling;WU Chunhong;CHEN Chanyou;WAN Heping;ZHAO Huixia(Hubei Province Engineering Research Center for Legume Plants,School of Life Sciences,Jianghan University,Wuhan 430056,China)

机构地区：[1]江汉大学生命科学学院,湖北省豆类(蔬菜)植物工程技术研究中心,武汉430056

出　　处：《园艺学报》2025年第1期111-122,共12页Acta Horticulturae Sinica

基　　金：湖北省教育厅科学研究计划项目(B2022283)。

摘　　要：捕光叶绿素a/b结合蛋白(light-harvesting chlorophyll a/b-binding protein,Lhc)直接影响光合效率和作物产量。为了系统研究长豇豆(Vigna unguiculata subsp.sesquipedalis)Lhc家族基因的特性及其在盐胁迫下的表达情况,以拟南芥Lhc蛋白序列为种子序列,并结合Lhc蛋白保守结构域PF00504,从长豇豆基因组中共鉴定了27个Lhc家族成员,并对其理化性质、基因结构、系统演化及共线性等进行分析。结果表明VuLhc启动子区光响应顺式作用元件数量远多于其他的元件,VuLhc可能参与长豇豆光调控过程,具有影响其生长发育的潜力。采用qRT-PCR检测了10个VuLhc在盐胁迫下的表达模式,结果显示其中9个呈上调趋势,其可能在豇豆对盐胁迫的响应中发挥重要作用。Light-harvesting chlorophyll a/b-binding proteins(Lhc)can directly affect photosynthetic efficiency and crop yield.To systematically study the characteristics of the Lhc gene family in long cowpea(Vigna unguiculata subsp.sesquipedalis)and their expression under salt stress,27 Lhc family members were identified from the cowpea genome using Arabidopsis Lhc protein sequences as seed sequences,combined with the conserved domain PF00504.Analyses were conducted on their physicochemical properties,gene structure,phylogenetic relationships,and synteny.The analysis indicated that the VuLhc promoter regions were enriched with light-responsive elements,significantly more than other cis-acting elements.This suggested that the VuLhc gene might be involved in the photoregulation of long cowpea,potentially influencing its growth and development.Additionally,qRT-PCR was employed to examine the expression patterns of 10 VuLhc genes under salt stress,revealing that 9 of them were upregulated,indicating their potential significant role in cowpea’s response to salt stress.

关 键 词：豇豆 捕光叶绿素a/b结合蛋白 VuLhc基因家族 表达模式 盐胁迫 

分 类 号：S634.4[农业科学—蔬菜学]