基于微量热法的果胶酶酶解烟叶果胶的热动力学研究  

Thermokinetic Study of Pectinase Hydrolysis of Pectin in Tobacco Based on Microcalorimetry

作　　者：李亚玲 张柯[1] 丁为 王兵[1] 付丽丽 周东 尚小利 杜薇 宋光富 王乐[1] 李玉娥 元野 乔学义[1] LI Yaling;ZHANG Ke;DING Wei;WANG Bing;FU Lili;ZHOU Dong;SHANG Xiaoli;DU Wei;SONG Guangfu;WANG Le;LI Yue;YUAN Ye;QIAO Xueyi(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Technology Center,China Tobacco Sichuan Industrial Co.,Ltd.,Chengdu 450000,China;Jilin Tobacco Industrial Co.,Ltd.,Changchun 130022,China;Tobacco Research Institute of Mudanjiang,Mudanjiang 157011,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州市高新技术产业开发区450001 [2]四川中烟工业有限责任公司技术中心,成都市锦江区610066 [3]吉林烟草工业有限责任公司,长春市经开区130022 [4]牡丹江烟草科学研究所,黑龙江省牡丹江市157011

出　　处：《中国烟草学报》2025年第1期66-75,共10页Acta Tabacaria Sinica

基　　金：中国烟草总公司重点科技项目“基于生物酶发酵技术的不适用烟叶价值提升研究与应用”(110202102004);“基于‘润甜香’品类导向的多极化配方技术研究与应用”(110202103007);“以重点品牌烟叶原料高效利用为导向的八大香型小叶组配方技术研究与应用”(110202103002)。

摘　　要：【目的】建立一种实时、动态检测外源酶催化烟叶水解反应的方法。【方法】利用微量热仪研究果胶酶催化烟叶水解反应的热动力学特性。【结果】果胶酶催化烟叶水解反应可分为两个阶段,I阶段是酶液与烟叶接触后,热流呈直线上升,达到峰值,II阶段是热流从峰值开始缓慢平稳下降,逐渐回到基线水平;通过热流-时间曲线,可以获得反应时间T、峰值P_(max)、放热量Q、反应速率常数k和反应进度φ等水解反应过程的热动力学参数;依据热流曲线及参数,能够较好地表征烟叶与酶制剂水解反应的差异。【结论】微量热法能够实时、动态、定性、定量表征果胶酶催化烟叶水解反应过程。[Objective]This study aims to establish a real-time and dynamic method for detecting the hydrolysis of tobacco catalyzed by exogenous enzymes.[Methods]The thermodynamic characteristics of pectinase-catalyzed hydrolysis of tobacco were studied by microcalorimeter.[Results]The results indicate that the pectinase-catalyzed hydrolysis of tobacco can be divided into two stages:Stage I,where the heat flow rises linearly to a peak after the enzyme solution contacts the tobacco leaves;and Stage II,where the heat flow gradually and steadily declines from the peak back to the baseline level.By analyzing the power-time curve,various thermodynamic parameters of the hydrolysis process can be obtained,including reaction time T,peak heat value P_(max),heat release Q,reaction rate constant k,and reaction progress.Furthermore,the differences in the hydrolysis reaction between tobacco leaves and enzyme preparations can be well characterized based on the heat flow curve and parameters.[Conclusion]The experimental results demonstrate that microcalorimetry can real-time,dynamically,qualitatively,and quantitatively characterize the hydrolysis reaction process of tobacco leaves catalyzed by pectinase.

关 键 词：微量热法 烟叶 酶 水解反应 热动力学 

分 类 号：TS2[轻工技术与工程—食品科学与工程]