京津冀地区肉鸡不同生产环节沙门菌的流行和传播  

Prevalence and Transmission of Salmonella Among Different Broiler Breeding Processes in Beijing-Tianjin-Hebei Region

作　　者：刘芳琴 崔明全 张璐[1] 陈玉 王鹤佳[1] 张纯萍[1] LIU FangQin;CUI MingQuan;ZHANG Lu;CHEN Yu;WANG HeJia;ZHANG ChunPing(China Institute of Veterinary Drug Control/Key Laboratory of Antimicrobial Resistance Monitoring of Animal-Derived Bacteria,Ministry of Agriculture and Rural Affairs,Beijing 100081;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,Shandong)

机构地区：[1]中国兽医药品监察所/农业农村部动物源细菌耐药性监测重点实验室,北京100081 [2]青岛农业大学动物医学院,山东青岛266109

出　　处：《中国农业科学》2025年第1期192-202,共11页Scientia Agricultura Sinica

基　　金：国家重点研发计划(2022YFD1800405,2016YFD0501600)。

摘　　要：【背景】沙门菌是重要的人畜共患病原菌和食源性病原菌,目前已发现2600多种血清型,不同血清型的沙门菌对动物和人的致病性不同。肉鸡是沙门菌流行传播的重要宿主之一,其污染状况直接关系着肉鸡的健康养殖和公共卫生安全,肉鸡生产链是肉鸡感染沙门菌的主要来源,主要涵盖了种鸡养殖、肉鸡孵化和肉鸡养殖等环节,研究沙门菌在肉鸡不同生产环节中的流行和传播情况对肉鸡沙门菌的防控和公共卫生安全具有重要意义。【目的】以京津冀地区肉种鸡养殖场、肉鸡孵化场和商品化肉鸡养殖场为切入点,系统研究肉鸡不同生产环节沙门菌的流行性、血清型分布和分子分型情况,为肉鸡生产中沙门菌流行和传播情况的研究提供参考。【方法】从北京、天津和河北地区肉鸡不同生产环节(包括同一养殖集团内部)的28个养殖场共采集样品2572份,其中肉种鸡养殖场、肉鸡孵化场和商品化肉鸡养殖场分别采集324份、747份和1501份样品。首先对采集样品进行沙门菌的增菌培养,然后用显色培养基和聚合酶链式反应(polymerase chain reaction,PCR)进行沙门菌的分离与鉴定;其次应用丹麦血清对已鉴定的沙门菌进行血清分型,并用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)方法对沙门菌进行分子分型;最后对不同生产环节养殖场肉鸡的带菌状况、血清型分布和分子分型结果进行分析比较。【结果】从肉鸡不同生产环节共分离沙门菌335株,总体分离率为13.0%,其中肉种鸡养殖场、肉鸡孵化场和商品化肉鸡养殖场沙门菌的分离率分别为3.4%、30.3%和6.5%。同一养殖集团内部不同生产环节共分离沙门菌32株,分离率为8.6%,其中肉种鸡养殖场、肉鸡孵化场和商品化肉鸡养殖场的沙门菌分离率分别为2.0%、14.0%和9.2%。沙门菌分离株共有11种血清型分布,优势血清型为肠炎,其次为田纳西、�【Background】Salmonella is an important zoonotic pathogen and foodborne pathogen,with over 2600 identified so far.Different serotypes of Salmonella exhibits varying degrees of pathogenicity to animals and humans.Broiler is one of the important hosts of Salmonella epidemic transmission,and its contamination status is directly related to the healthy breeding of broilers and public health security.The broiler production chain is the main source of Salmonella infection in broilers,which mainly covers breeder farms,broiler hatcheries,and broiler farms,etc.The study of the prevalence and transmission of Salmonella in different production links of broilers is of great significance for the prevention and control of Salmonella in broilers and for public health and safety.It is important to study the prevalence and transmission of Salmonella in different links of broiler production for the prevention and control of Salmonella in broiler and public health safety.【Objective】This study systematically investigated the prevalence,serotype distribution and molecular typing of Salmonella in different production chains of broilers,taking broiler breeder farms,broiler hatcheries and commercial broiler farms in the Beijing-Tianjin-Hebei region as an entry point,which provided data for the study of Salmonella epidemic and transmission in broiler production chain.【Method】A total of 2572 samples were collected from 28 broiler farms in different production stages of broilers in the Beijing-Tianjin-Hebei region(including within a certain breeding group),of which 324,747 and 1501 samples were collected from broiler breeder farms,broiler hatcheries and commercial broiler farms,respectively.Firstly,the samples were cultured for the enrichment of Salmonella,and then the isolation and identification of Salmonella were carried out by color development medium and Polymerase Chain Reaction(PCR);secondly,the identified Salmonella were serotyped by Danish serum,and the identification of Salmonella was carried out by Pulsed Field Gel Ele

关 键 词：肉鸡 沙门菌 生产环节 血清型 PFGE 

分 类 号：S858.31[农业科学—临床兽医学]