电针阳陵泉和曲池穴调控JAK2/STAT3信号通路改善大鼠脑卒中肢体痉挛  

Electroacupuncture at Yanglingquan and Quchi Points in Regulates the JAK2/STAT3 Signaling Pathway to Improve the Post-Stroke Spasticity in Rats

作　　者：何鑫[1,2] 赵健[3] 景伟[2] 刘敏 刘昱晗 韩鹏 HE Xin;ZHAO Jian;JING Wei;LIU Min;LIU Yuhan;HAN Peng(The First Clinical Medical School of Heilongjiang University of Chinese Medicine,Harbin 150000 Heilongjiang,China;The Second Hospital Affiliated to Heilongjiang University of Chinese Medicine,Harbin 150000 Heilongjiang,China;The Fourth Hospital Affiliated to Heilongjiang University of Chinese Medicine,Harbin 150000 Heilongjiang,China;The Second Hospital of Harbin,Harbin 150000 Heilongjiang,China;Heilongjiang University of Chinese Medicine,Harbin 150000 Heilongjiang,China)

机构地区：[1]黑龙江中医药大学第一临床医学院,黑龙江哈尔滨150000 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150000 [3]黑龙江中医药大学附属第四医院,黑龙江哈尔滨150000 [4]哈尔滨市第二医院,黑龙江哈尔滨150000 [5]黑龙江中医药大学,黑龙江哈尔滨150000

出　　处：《广州中医药大学学报》2025年第2期430-436,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：黑龙江省卫生健康委科研课题(编号:20230303100276)。

摘　　要：【目的】观察电针阳陵泉和曲池穴对大鼠脑卒中肢体痉挛的治疗作用及机制。【方法】将大鼠随机分为假手术组、模型组、电针组和电针+CA1[Janus激酶2(JAK2)激动剂]组。除假手术组,其余各组大鼠采用改良的大脑中动脉线栓联合内囊注射N-甲基-D-天冬氨酸(NMDA)受体法制备脑卒中肢体痉挛模型。干预后,对大鼠进行神经功能缺损评分、阿什沃斯肌肉痉挛评定量表(Ashworth)评分和电生理测定;酶联免疫吸附分析(ELISA)检测脑组织中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、γ-氨基丁酸(GABA)水平;采用比色法检测谷氨酸(Glu)含量;苏木素-伊红(HE)染色法观察脑组织病理学变化;Western Blot法检测脑组织中JAK2、磷酸化JAK2(p-JAK2)、信号转导子和转录激活子3(STAT3)、磷酸化STAT3(p-STAT3)蛋白表达。【结果】模型组神经功能缺损评分,Ashworth评分,脑组织中IL-6、TNF-α、Glu含量及p-JAK2/JAK2、p-STAT3/STAT3比值均显著高于假手术组(P<0.05),肌张力信号值、脑组织中GABA含量显著低于假手术组(P<0.05);与模型组比较,电针组神经功能缺损评分,Ashworth评分,脑组织中IL-6、TNF-α、Glu含量及p-JAK2/JAK2、p-STAT3/STAT3比值均显著降低(P<0.05),肌张力信号值、脑组织中GABA含量显著升高(P<0.05);与电针组比较,电针+CA1组神经功能缺损评分,Ashworth评分,脑组织中IL-6、TNF-α、Glu含量及p-JAK2/JAK2、p-STAT3/STAT3比值显著升高(P<0.05),肌张力信号值、脑组织中GABA含量显著降低(P<0.05)。【结论】电针可能通过抑制JAK2/STAT3通路抑制炎症反应,调节神经元兴奋/抑制平衡,从而缓解脑卒中大鼠肢体痉挛。Objective To observe the therapeutic effect and mechanism of electroacupuncture at Yanglingquan(GB34)and Quchi(LI11)points for post-stroke spasticity(PSS)in rats.Methods The rats were randomly divided into sham-operated group,model group,electroacupuncture group and electroacupuncture+CA1[Janus kinase 2(JAK2)agonist]group.The PSS model was prepared by the modified middle cerebral artery occlusion combined with internal capsule injection of N-methyl-D-aspartate(NMDA)receptor method in all groups of rats except the sham-operated group.After the intervention,the neurological deficit scale scoring,Ashworth Spasticity Scale(Ashworth)scoring and electrophysiological determination were carried out;the levels of interleukin 6(IL-6),tumor necrosis factorα(TNF-α)andγ-aminobutyric acid(GABA)in the brain tissues were detected by enzymelinked immunosorbent assay(ELISA);the content of glutamate(Glu)was observed by colorimetry;the pathological changes in brain tissues were observed by hematoxylin-eosin(HE)staining method;the protein expression levels of JAK2,phosphorylated JAK2(p-JAK2),signal transducer and activator of transcription 3(STAT3),and phosphorylated STAT3(p-STAT3)in brain tissues were detected by Western Blot method.Results The neurological deficit scale scores,Ashworth scores,contents of IL-6,TNF-αand Glu as well as p-JAK2/JAK2 and p-STAT3/STAT3 ratios in brain tissues in the model group were all significantly higher than those in the shamoperated group(P<0.05),and the muscular tension signal value and GABA content in brain tissues in the model group were significantly lower than those in the sham-operated group(P<0.05);compared with the model group,the neurological deficit scale scores,Ashworth scores,contents of IL-6,TNF-αand Glu as well as p-JAK2/JAK2 and p-STAT3/STAT3 ratios in brain tissues were significantly lower in the electroacupuncture group(P<0.05),and the muscular tension signal value and GABA content in brain tissues were significantly higher in the electroacupuncture group(P<0.05);compared with t

关 键 词：电针 脑卒中肢体痉挛 阳陵泉穴 曲池穴 JAK2/STAT3信号通路 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]