十敛散联合舒脉胶囊通过调控PI3K/AKT/Relaxin/Apelin通路促进糖尿病足溃疡大鼠创面愈合  

Efficacy of Shilian Powder Combined with Shumai Capsules in Promoting Wound Healing in Rats with Diabetic Foot Ulcers through Regulating the PI3K/Akt/Relaxin/Apelin Pathway

作　　者：曾艳平 邵紫欣 莫伟[1,2] 刘阳 李天昊[3] 吕雄 毕建璐[1,2] ZENG Yanping;SHAO Zixin;MO Wei;LIU Yang;LI Tianhao;LYU Xiong;BI Jianlu(The Fifth Clinical Medical School of Guangzhou University of Chinese Medicine,Guangzhou 510095 Guangdong,China;Guangdong Second Traditional Chinese Medicine Hospital,Guangzhou 510095 Guangdong,China;Guangzhou Hospital of Intergrated Traditonal and West Medicine Affiliated to Guangzhou University of Chinese Medicine,Guangzhou 510800 Guangdong,China)

机构地区：[1]广州中医药大学第五临床医学院,广东广州510095 [2]广东省第二中医院,广东广州510095 [3]广州中医药大学附属广州市中西医结合医院,广东广州510800

出　　处：《广州中医药大学学报》2025年第2期461-468,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省第二中医院中医特色重点医院建设工程项目(编号:602023069);广州市科技计划项目(编号:201904010194)。

摘　　要：【目的】观察外用十敛散联合内服舒脉胶囊对糖尿病足溃疡(DFU)大鼠的治疗作用及机制。【方法】将足溃疡造模成功的雄性大鼠8只设为对照组。将DFU造模成功的雄性大鼠24只随机分为DFU组、舒脉组、十敛散合舒脉组,每组8只。对应干预后,比较各组大鼠的创面愈合率,创面组织病理学变化,血清中炎症因子白细胞介素(IL)-6、IL-1β和血管内皮生长因子(VEGF)水平,创面组织松弛素(Relaxin)、爱帕琳肽(Apelin)、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)、VEGF蛋白表达水平,PI3K、AKT、Relaxin和Apelin mRNA表达水平。【结果】与对照组比较,DFU组创面愈合率,血清、创面VEGF水平,创面Relaxin水平,创面AKT蛋白、mRNA水平均显著降低(P<0.05),血清IL-6、IL-1β水平,创面Apelin水平,PI3K蛋白、mRNA表达水平均显著升高(P<0.05),镜下可见创面肉芽组织及新生的毛细血管减少,炎性细胞浸润增多。与DFU组比较,舒脉组和十敛散合舒脉组创面愈合率,血清、创面VEGF水平,创面Relaxin、Apelin水平,创面PI3K和AKT的蛋白及mRNA表达水平均显著升高(P<0.05),血清IL-6、IL-1β水平均显著降低(P<0.05),镜下可见创面肉芽组织及新生的毛细血管增多,炎性细胞浸润减少。与舒脉组比较,十敛散合舒脉组创面愈合率,创面VEGF水平,创面Relaxin、Apelin水平,创面PI3K和AKT的蛋白及mRNA水平均显著升高(P<0.05),血清IL-6、IL-1β水平均显著降低(P<0.05),镜下可见创面肉芽组织及新生的毛细血管增多,炎性细胞浸润减少。【结论】外用十敛散联合内服舒脉胶囊可促进糖尿病足溃疡大鼠创面愈合,其机制与激活PI3K/AKT/Relaxin/Apelin信号通路有关。Objective To observe the therapeutic effect and mechanism of external application with Shilian Powder combined with oral administration of Shumai Capsules for the treatment of rats with diabetic foot ulcer(DFU).Methods Eight male rats with successfully modeled foot ulcer(DF)were used as the control group.While 24 male rats with successfully modeled DFU were randomly divided into DFU group,Shumai Capsules group and Shilian Powder combined with Shumai Capsules group,with eight rats in each group.After the corresponding interventions,we determined the wound healing rate,histopathological changes of wound,levels of inflammatory factors such as interleukin(IL)-6,IL-1βand vascular endothelial growth factor(VEGF)in serum,levels of Apelin and Relaxin,and protein expressions of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT)and VEGF in wound tissue,as well as mRNA expressions of PI3K,AKT,Relaxin and Apelin.Results Compared with the control group,the DFU group showed a significant decrease in wound healing rate,VEGF level in serum and wound,wound Relaxin level,protein and mRNA levels of wound AKT(P<0.05),and a significant increase in serum IL-6 and IL-1βlevels,wound Apelin level,wound PI3K protein and mRNA levels(P<0.05),and the reduced wound granulation tissue and formation of new capillaries and increased inflammatory cell infiltration were seen under the microscope.Compared with the DFU group,the wound healing rate,VEGF level in serum and wound,wound Relaxin and Apelin levels,protein and mRNA levels of wound PI3K and AKT in the Shumai Capsules group and Shilian Powder combined with Shumai Capsules group were significantly increased(P<0.05),and serum IL-6 and IL-1βlevels were significantly decreased(P<0.05),and the increased wound granulation tissue and formation of new capillaries and reduced inflammatory cell infiltration were seen under the microscope.Compared with the Shumai Capsules group,the wound healing rate,wound VEGF level,wound Relaxin and Apelin levels,protein and mRNA levels of wound PI3K and AK

关 键 词：十敛散 舒脉胶囊 糖尿病足溃疡 创面愈合 炎症反应 血管生成 PI3K/AKT/Relaxin/Apelin信号通路 大鼠 

分 类 号：R285.5[医药卫生—中药学]