木香显微特征指数与化学成分的相关性研究  

Study on the Correlation Between Microscopic Features Indexes and Chemical Constituents of Aucklandiae Radix

作　　者：崔朋 李桃 陈绍静 代娅飞 李昌茜 和振全 俞捷 李学芳 CUI Peng;LI Tao;CHEN Shaojing;DAI Yafei;LI Changxi;HE Zhengquan;YU Jie;LI Xuefang(Yunnan University of Chinese Medicine,Kunming 650500 Yunnan,China;Yulong Naxi Autonomous County Ludian Shanyun Pharmaceutical Co.,Ltd.,Lijiang 674100 Yunnan,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]玉龙纳西族自治县鲁甸山芸药业有限责任公司,云南丽江674100

出　　处：《广州中医药大学学报》2025年第2期469-476,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：云南省科技重大专项——纳西族特色民族医药挖掘整理与研究开发(编号:202302AA310014)。

摘　　要：【目的】分析木香横切面油室数量、韧皮部占比与木香烃内酯和去氢木香内酯含量的相关性,及粉末特征中菊糖数、导管数与多糖含量的相关性,探索木香“辨状论质”的科学依据。【方法】采用横切面永久制片法、水合氯醛装片法、计数分析法对木香油室、菊糖和导管的显微特征常数值进行测定,分别采用高效液相色谱法和苯酚-硫酸法对木香烃内酯和去氢木香内酯含量、多糖含量进行测定,将两组数据用统计方法进行相关性分析。【结果】木香横切面单位面积油室数、韧皮部单位面积油室数与木香烃内酯和去氢木香内酯含量之间有显著的正相关关系;木香粉末中菊糖数与木香多糖含量存在显著的正相关关系;导管数与多糖含量之间无相关关系。【结论】初步建立了一种基于木香显微特征常数测定的中药材质量评价新方法及新技术,可为木香的质量控制提供新的技术手段。Objective To analyze the correlation between the number of oil chambers,the proportion of phloem at cross-section of Aucklandiae Radix and the contents of costunolide and dehydrocostuslactone,and the correlation between inulin quantity,vessel quantity and polysaccharide content in powder characteristics,so as to explore the scientific basis for Aucklandiae Radix“identifying the quality based on appearance differentiation”.Methods The constant values of the microscopic features of oil chambers,inulin and vessels were determined by cross-sectional permanent slice method,chloral hydrate method and counting analysis method,and the contents of costunolide and dehydrocostuslactone as well as polysaccharide content were determined by the highperformance liquid chromatography(HPLC)and the phenol-sulfuric acid method,respectively,and the correlation in the two groups of data was analyzed by statistical methods.Results There was a significantly positive correlation between the number of oil chambers per unit area in the cross-section of Aucklandiae Radix and the number of oil chambers per unit area in the phloem and the contents of costunolide and dehydrocostuslactone;there was a significantly positive correlation between the quantity of inulin in the powder of Aucklandiae Radix and the polysaccharide content;there was no correlation between the quantity of vessels and the polysaccharide content.Conclusion This study initially established a new method and a new technology for quality evaluation of Chinese herbal medicines based on the determination of microscopic characteristic constants of Aucklandiae Radix,which can provide a new technical method for the quality control of Aucklandiae Radix.

关 键 词：木香 显微定量 指标性成分含量 多糖含量 相关性分析 质量控制 

分 类 号：R282.5[医药卫生—中药学]