Characterization and genomic analysis of Pseudoalteromonas phage v B_Pal P_Y7,representing a novel viral genus,Miuvirus  

作　　者：Miaolan WU Hongmin WANG Ye MI Yantao LIANG Kaiyang ZHENG Yundan LIU Yue DONG Linyi REN Yue SU Hongbing SHAO Yeong Yik SUNG Wen Jye MOK Li Lian WONG Min WANG 

机构地区：[1]College of Marine Life Sciences,Institute of Evolution and Marine Biodiversity,MoE Laboratory of Evolution and Marine Biodiversity,Frontiers Science Center for Deep Ocean Multispheres and Earth System,Center for Ocean Carbon Neutrality,Ocean University of China,Qingdao 266100,China [2]Qingdao City Center for Disease Control and Prevention,Qingdao Institute of Prevention Medicine,Qingdao 266033,China [3]UMT-OUC Joint Centre for Marine Studies,Qingdao 266100,China [4]Institute of Marine Biotechnology,Universiti Malaysia Terengganu,Kuala Terengganu 21030,Malaysia [5]Haide College,Ocean University of China,Qingdao 266100,China [6]The Affiliated Hospital of Qingdao University,Qingdao 266000,China

出　　处：《Journal of Oceanology and Limnology》2025年第1期299-312,共14页海洋湖沼学报(英文)

基　　金：the National Natural Science Foundation of China(Nos.42188102,42120104006,41976117,42176111);the Fundamental Research Funds for the Central Universities(Nos.202172002,201812002);the funding from Andrew Mc Minn。

摘　　要：Pseudoalteromonas is a group of marine bacteria widespread in diverse marine sediments,producing a wide range of bioactive compounds.However,only a limited number of Pseudoalteromonas phages have been isolated and studied.In this study,a novel lytic Pseudoalteromonas phage,denoted as vB_PalP_Y7,was isolated from sewage samples collected at the Seafood Market in Qingdao,China.vB_PalP_Y7 remained stable across a wide range of temperatures(-20–50℃)and a wide pH range(3–12).The vB_PalP_Y7 phage harbors a linear double-stranded DNA molecule of 57699 base pairs(bp)with a G+C content of 45.90%.Furthermore,it is predicted to contain 58 open reading frames(ORFs).Phylogenetic analysis and protein network relationship analysis revealed low similarity between vB_PalP_Y7 and viruses in the ICTV and IMG/VR4 database,suggesting that vB_PalP_Y7 may be a potential new genus,Miuvirus.This study contributed valuable insights to comprehend the relationship between Pseudoalteromonas phages and their host organisms.

关 键 词：Pseudoalteromonas phage genomic analysis phylogenetic analysis viral cluster 

分 类 号：Q938[生物学—微生物学]