Dyssynchronous heart failure models in canines:New insights into electrocardiographic,echocardiographic and histological features  

作　　者：Han Jin Shengwen Yang Hao Huang Sijing Cheng Pengkang He Sixian Weng Min Gu Hongxia Niu Wei Hua Yiran Hu Hui Li 

机构地区：[1]Cardiology Department,Peking University First Hospital,Beijing,China [2]Department of Heart Center,Beijing Chaoyang Hospital,Capital Medical University,Beijing,China [3]Cardiac Arrhythmia Center,Fuwai Hospital,State Key Laboratory of Cardiovascular Disease,National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China [4]Department of Cardiology and Macrovascular Disease,Beijing Tiantan Hospital,Capital Medical University,Beijing,China [5]Department of Ultrasound,State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China

出　　处：《Animal Models and Experimental Medicine》2025年第1期142-153,共12页动物模型与实验医学(英文)

基　　金：This work was financially supported by National Natural Science Foundation of China(Nos.82000325,82100325,82070349);Young Elite Scientists Sponsorship Program by Beijing Association for Science and Technology(No.BYESS2023392);The Beijing Gold-bridge project(No.ZZ21055);The Peking University First Hospital Seed Foundation(No.2020SF19);High-level hospital clinical research funding of Fuwai Hospital,Chinese Academy of Medical Sciences(No.2022-GSP-GG-11).

摘　　要：Background:We investigated the similarities and differences between two experimental approaches using tachy-pacing technology to induce desynchronized heart failure in canines.Methods:A total of eight dogs were included in the experiment,four were tachypaced in right ventricle apex (RVAP) and 4 were paced in right atrium after the ablation of left bundle branch to achieve left bundle branch block (RAP+LBBB).Three weeks of follow-up were conducted to observe the changes in cardiac function and myocardial staining was performed at the end of the experiment.Results:Both experimental approaches successfully established heart failure with reduced ejection fraction models,with similar trends in declining cardiac function.The RAP+LBBB group exhibited a prolonged overall ventricular activation time, delayed left ventricular activation,and lesser impact on the right ventricle.The RVAP approach led to a reduction in overall right ventricular compliance and right ve ntricular enlargement.The RAP+LBBB group exhibited significant reductions in left heart compliance (LVGLS,%:RAP+LBBB-12.60±0.12 to-5.93±1.25;RVAP-13.28±0.62to-8.05±0.63, p=0.023;LASct,%:RAP+LBBB-15.75±6.85 to-1.50±1.00;RVAP-15.75±2.87 to-10.05±6.16,p=0.035).Histological examination revealed more pronounced fibrosis in the left ventricular wall and left atrium in the RAP+LBBBgroup while the RVAP group showed more prominent fibrosis in the right ventricular myocardium.Conclusion:Both approaches establish HFrEF models with comparable trends.The RVAP group shows impaired right ventricular function,while the RAP+LBBB group exhibits more severe decreased compliance and fibrosis in left ventricle.

关 键 词：DYSSYNCHRONY heart failure tachy-pacing technology 

分 类 号：R54[医药卫生—心血管疾病]