基于中空金增敏的免疫层析技术用于化妆品中非法添加雌激素的检测研究  

Study on the Detection of Illegal Addition of Estrogen in Cosmetics Using Immunochromatography Assay Technology Based on Hollow Gold Nanoparticles

作　　者：刘晓瑜 何星月 沈倩 沈雁[2] 沈于兰 陈国清 LIU Xiao-yu;HE Xing-yue;SHEN Qian;SHEN Yan;SHEN Yu-lan;CHEN Guo-qing(Wuxi Center for Drug Safety Control,Wuxi 214028,China;School of Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]无锡市药品安全检验检测中心,江苏无锡214028 [2]中国药科大学药学院,江苏南京211198

出　　处：《分析测试学报》2025年第2期294-301,共8页Journal of Instrumental Analysis

基　　金：江苏省市场监督管理局科技计划项目(KJ2024068)。

摘　　要：该文基于中空金增敏的免疫层析技术,将中空金纳米粒应用于化妆品中非法添加雌激素的检测研究,构建了一种检测化妆品中雌二醇的中空金免疫层析试纸条。优化了金标抗体标记pH值、标记抗体量、金标抗体添加量及金标垫处理液等条件。结果显示:在最优条件下,建立的中空金免疫层析法对雌二醇的检出限为10 ng/mL,线性范围为0.01~10 ng/mL。该方法的检测特异性良好,面膜液中雌二醇的加标回收率为89.0%~107%。该试纸条具有比胶体金显色法更低的检出限和较好的特异性,且检测速度快,适用于化妆品中雌二醇非法添加的现场大规模初筛。In this paper,immunochromatographic test strip(HGNPs-ICTS)based on hollow gold nanoparticles(HGNPs)for qualitative detection of estradiol in cosmetics was constructed.The effects of pH value,the amount of gold-labeled antibody,the addition amount of labeled antibody and the gold-labeled pad buffers were carefully investigated.The results showed that under the optimal condi⁃tions,the detection limit of estradiol was 10 ng/mL,and the linear range was 0.01-10 ng/mL.The detection specificity was good,and the recovery of estradiol was 89.0%-107%.HGNPs-ICTS has lower detection limit and better specificity than colloidal gold immunochromatographic assay(GICA),and the detection speed is fast.It is suitable for large-scale on-the-spot screening of estradiol illegally added in cosmetics.

关 键 词：中空金 免疫层析技术 化妆品 非法添加 激素检测 快速筛查 

分 类 号：O657[理学—分析化学] TQ658[理学—化学]