丹黄散含药血清对高糖诱导血管内皮细胞损伤的修复作用  

Repair Effect of Danhuang Powder-Containing Serum on High Glucose-Induced Vascular Endothelial Cell Injury

作　　者：赵思思 张春玲 赵伟 邸铁涛 周世勇 陈露 魏良纲[3] 张艳 董源源 范燚 朱蕾 罗智钦 王兴辉 ZHAO Sisi;ZHANG Chunling;ZHAO Wei;DI Tietao;ZHOU Shiyong;CHEN Lu;WEI Lianggang;ZHANG Yan;DONG Yuanyuan;FAN Yi;ZHU Lei;LUO Zhiqin;WANG Xinghui(Guizhou University of Traditional Chinese Medicine,Guizhou 550002 Guiyang,China;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guizhou 550003 Guiyang,China;Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China)

机构地区：[1]贵州中医药大学,贵州贵阳550002 [2]贵州中医药大学第二附属医院,贵州贵阳550003 [3]重庆市中医院,重庆400021

出　　处：《广州中医药大学学报》2025年第1期185-190,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81960805)。

摘　　要：【目的】观察丹黄散含药血清对高糖诱导的血管内皮细胞损伤的修复作用及其机制。【方法】制备丹黄散含药血清。培养人脐静脉血管内皮细胞(HUVECs),将其分为对照组、重组人表皮生长因子(简称生长因子)组、丹黄散组、高糖组、高糖+生长因子组、高糖+丹黄散组。各组对应处理48 h后,应用透射电镜观察细胞超微结构及自噬情况,流式细胞仪检测细胞凋亡,Western Blot法检测细胞中血管内皮生长因子(VEGF)、表皮生长因子(EGF)和碱性成纤维细胞生长因子(bFGF)的蛋白表达水平。【结果】(1)对照组细胞线粒体内脊清晰可见,自噬体及自噬溶酶体较少见;高糖组线粒体肿胀、不规则,出现空泡化;高糖+丹黄散组可见较多典型自噬样结构。(2)与高糖组、高糖+生长因子组比较,高糖+丹黄散组细胞凋亡率显著降低(P <0.05)。(3)与高糖组、高糖+生长因子组比较,高糖+丹黄散组细胞中VEGF、EGF、bFGF的蛋白表达水平显著升高(P <0.05)。【结论】丹黄散含药血清可减轻高糖对HUVEC细胞的损伤,其机制可能与激活线粒体自噬,抑制细胞凋亡以及上调VEGF、EGF、bFGF的表达有关。Objective To observe the repair effect and mechanism of Danhuang Powder-containing serum on high glucose-induced vascular endothelial cell injury.Methods Danhuang Powder-containing serum was prepared.Human umbilical vein endothelial cells(HUVECs)were cultured to be divided into control group,recombinant human epidermal growth factor(called“growth factor”for short)group,Danhuang Powder group,high glucose group,high glucose+growth factor group,and high glucose+Danhuang Powder group.After corresponding intervention in each group for 48 hours,the cell ultrastructure and autophagy were observed under transmission electron microscope,apoptosis was detected by flow cytometry,and the protein expression levels of vascular endothelial growth factor(VEGF),epidermal growth factor(EGF)and basic fibroblast growth factor(bFGF)in the cells were detected by Western Blot.Results(1)The intra-mitochondrial ridges in the control group were clearly visible,autophagosomes and autolysosomes were fewer;mitochondria in the high glucose group were swollen and irregular,and appeared vacuolated;and the more typical autophagy-like structures were seen in the high glucose+Danhuang Powder group.(2)Compared with the high glucose group and high glucose+growth factor group,the apoptosis rate of cells in the high glucose+Danhuang Powder group was significantly decreased(P<0.05).(3)Compared with the high glucose group and the high glucose+growth factor group,the protein expression levels of VEGF,EGF and bFGF in the cells of the high glucose+Danhuang Powder group were significantly increased(P<0.05).Conclusion Danhuang Powder-containing serum can reduce the high glucose-induced damage in HUVEC cells,and its mechanism may be related to the activation of mitochondrial autophagy,and the inhibition of apoptosis,as well as the up-regulation of the expression of VEGF,EGF and bFGF.

关 键 词：丹黄散 糖尿病足溃疡 自噬 凋亡 血管内皮生长因子 表皮生长因子 碱性成纤维细胞生长因子 人脐静脉血管内皮细胞 

分 类 号：R285.5[医药卫生—中药学]