右美托咪定通过转化生长因子-β1途径对大鼠脊柱切除术后硬膜外纤维化的影响  

Effect of dexmedetomidine on epidural fibrosis afterspinal surgery in rats via the transforming growthfactor-β1 pathway

作　　者：侯国清 岳海龙 常倩 马俊涛 HOU Guoqing;YUE Hailong;CHANG Qian;MA Juntao(Department of Anesthesiology,Tangshan Central Hospital of Hebei Province,Tangshan,Hebei,063700;Hemodialysis Room,Tangshan Central Hospital of Hebei Province,Tangshan,Hebei,063700;Department of Critical Care Medicine,Tangshan Central Hospital of Hebei Province,Tangshan,Hebei,063700)

机构地区：[1]河北省唐山中心医院麻醉科,河北唐山063700 [2]河北省唐山中心医院血液透析室,河北唐山063700 [3]河北省唐山中心医院重症医学科,河北唐山063700

出　　处：《实用临床医药杂志》2025年第1期77-82,共6页Journal of Clinical Medicine in Practice

基　　金：河北省2021年度医学科学研究基金课题(20211486)。

摘　　要：目的 探讨右美托咪定通过转化生长因子-β1(TGF-β1)信号通路对大鼠脊柱切除术后硬膜外纤维化的影响。方法 选取40只大鼠,将10只大鼠设为对照组;其余30只构建脊柱切除术后模型,并随机分为模型组、右美托咪定组、右美托咪定+TGF-β1激动剂组,每组10只,剔除建模失败大鼠,最终每组各有9只大鼠纳入后续研究。观察并比较各组大鼠硬膜组织的病理学特征、成纤维细胞数量、PCNA阳性细胞表达率、炎症反应指标[白细胞介素(IL)-6、IL-1β、IL-8]水平、应激反应指标[丙二醛(MDA)、超氧化物歧化酶(SOD)]水平及TGF-β1信号通路相关mRNA与蛋白表达量。结果 与对照组比较,另外3组的成纤维细胞数量均增加,PCNA阳性细胞表达率均升高,IL-6、IL-1β、IL-8、MDA水平均升高,SOD水平均降低,TGF-β1 mRNA、Smad3 mRNA表达量和TGF-β1、Smad3蛋白表达量均升高,差异有统计学意义(P<0.05);与模型组比较,右美托咪定组、右美托咪定+TGF-β1激动剂组的成纤维细胞数量均减少,PCNA阳性细胞表达率均降低,IL-6、IL-1β、IL-8、MDA水平均降低,SOD水平均升高,TGF-β1 mRNA、Smad3 mRNA表达量和TGF-β1、Smad3蛋白表达量均降低,差异有统计学意义(P<0.05);与右美托咪定组比较,右美托咪定+TGF-β1激动剂组的成纤维细胞数量增加,PCNA阳性细胞表达率升高,IL-6、IL-1β、IL-8、MDA水平升高,SOD水平降低,TGF-β1 mRNA、Smad3 mRNA表达量和TGF-β1、Smad3蛋白表达量升高,差异有统计学意义(P<0.05)。结论 右美托咪定可通过抑制TGF-β1信号通路,显著减轻大鼠脊柱切除术后的炎症反应和应激反应,减少术后硬膜外纤维化。Objective To investigate the impact of dexmedetomidine on epidural fibrosis after spinal surgery in rats through the transforming growth factor-β1(TGF-β1)signaling pathway.Methods A total of 40 rats were selected,with 10 rats assigned to control group.The remaining 30 rats underwent spinal surgery modeling and were randomly divided into model group,dexmedetomidine group,and dexmedetomidine+TGF-β1 agonist group,with 10 rats in each.Rats with unsuccessful modeling were excluded,resulting in 9 rats in each group for subsequent analysis.Pathological characteristics of epidural scar tissue,fibroblast count,PCNA-positive cell expression rate,levels of inflammatory markers[interleukin(IL)-6,IL-1β,IL-8],stress response indicators[malondialdehyde(MDA),superoxide dismutase(SOD)],and expression levels of mRNAs and proteins related to the TGF-β1 signaling pathway were observed and compared among the groups.Results Compared with the control group,the other three groups showed increased fibroblast count,elevated PCNA-positive cell expression rate,higher levels of IL-6,IL-1β,IL-8,and MDA,lower SOD levels,and increased expression levels of TGF-β1 mRNA,Smad3 mRNA,and TGF-β1 and Smad3 proteins,with statistically significant differences ( P <0.05). Compared with the model group , both the dexmedetomidinegroup and the dexmedetomidine + TGF-β1 agonist group exhibited decreased fibroblast count, reduced PCNA-positive cell expression rate, lower levels of IL-6, IL-1β, IL-8 and MDA, higher SOD levels, and decreased expression levels of TGF-β1 mRNA, Smad3 mRNA, and TGF-β1 and Smad3 proteins, with statistically significant differences ( P <0.05). Compared with the dexmedetomidine group, the dexmedetomidine + TGF-β1 agonist group showed increased fibroblast count, elevated PCNA-positive cell expression rate, higher levels of IL-6, IL-1β, IL-8 and MDA, lower SOD levels, and increased expression levels of TGF-β1 mRNA, Smad3 mRNA, and TGF-β1 and Smad3 proteins, with statistically significant differences ( P <0.05). Conclusion

关 键 词：脊柱切除术 右美托咪定 转化生长因子-Β1 硬膜外纤维化 大鼠 

分 类 号：R687[医药卫生—骨科学] R614.2[医药卫生—外科学] R446[医药卫生—临床医学]