解脂耶氏酵母生物合成芳香族氨基酸衍生物的合成生物学策略  

Strategy for the biosynthesis of aromatic amino acid derivatives byYarrowia lipolytica

作　　者：陈茁 朱源 李伟国 闫晓光 颜丙扬 乔建军[1,2] 赵广荣[1,2] CHEN Zhuo;ZHU Yuan;LI Weiguo;YAN Xiaoguang;YAN Bingyang;QIAO Jianjun;ZHAO Guangrong(Frontiers Science Center for Synthetic Biology,Key Laboratory of Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China;Zhejiang Institute of Tianjin University,Shaoxing 312300,Zhejiang,China)

机构地区：[1]天津大学化工学院、教育部合成生物学前沿科学中心、系统生物工程教育部重点实验室,天津300350 [2]天津大学浙江研究院(绍兴),浙江绍兴312300

出　　处：《微生物学通报》2025年第1期33-45,共13页Microbiology China

基　　金：天津大学浙江研究院(绍兴)自主基金(2023X2-0013)。

摘　　要：芳香族氨基酸衍生物广泛存在于自然界中,具有多种生物活性,包括抗氧化、抗炎、神经保护和抗癌特性。在食品、化妆品、营养保健品、化学等领域,尤其在制药工业中发挥着重要作用。近年来,解脂耶氏酵母(Yarrowia lipolytica)作为一种非常规模式生物,因其独特的生理生化特性在生物合成领域展现出独特的潜力,特别是在芳香族氨基酸衍生物的合成上,包括白藜芦醇和柚皮素等。由于其独特的胞质环境和高通量的乙酰辅酶A,解脂耶氏酵母成为合成芳香族氨基酸衍生物的理想宿主。本文总结了解脂耶氏酵母在生产芳香族氨基酸衍生物方面的合成生物学策略,并汇总目前解脂耶氏酵母合成芳香族氨基酸衍生物的进展,对解脂耶氏酵母底盘细胞在合成生物学未来的发展和挑战进行了展望。Aromatic amino acid derivatives are widely distributed in the nature and possess antioxidant,anti-inflammatory,neuroprotective,and anticancer activities.They play important roles in various fields such as food,cosmetics,nutritional supplements,and chemistry,particularly in the pharmaceutical industry.In recent years,the unconventional model organism Yarrowia lipolytica has emerged as a promising candidate in biosynthesis due to its unique physiological and biochemical characteristics.Specifically,it has shown significant potential in the synthesis of aromatic amino acid derivatives,including resveratrol and naringenin.With a distinctive cytosolic environment and high flux of acetyl-CoA,Y.lipolytica stands out as an ideal host for the synthesis of aromatic amino acid derivatives.This review summarizes the biosynthesis strategies of Y.lipolytica in the production of aromatic amino acid derivatives and reviews the current progress in this field.Furthermore,it discusses the future prospects and challenges of applying Y.lipolytica as a chassis cell in Synthetic Biology.

关 键 词：解脂耶氏酵母 芳香族氨基酸衍生物 合成生物学 代谢工程 

分 类 号：Q819[生物学—生物工程]