检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:Fernando H.Biase Sarah E.Moorey Julie G.Schnuelle Soren Rodning Martha Sofia Ortega Thomas E.Spencer
机构地区:[1]School of Animal Sciences,Virginia Polytechnic Institute and State University,175 W Campus Dr,Blacksburg,VA 24061,USA [2]Department of Animal Science,University of Tennessee,Knoxville,TN 37996,USA [3]Department of Clinical Sciences,Auburn University,Auburn,AL 36849,USA [4]Department of Animal Science,Auburn University,Auburn,AL 36849,USA [5]Department of Animal and Dairy Sciences,University of Wisconsin Madison,Madison,WI 53706,USA [6]Division of Animal Sciences,University of Missouri,Columbia,MO 65211,USA
出 处:《Journal of Animal Science and Biotechnology》2025年第1期76-91,共16页畜牧与生物技术杂志(英文版)
基 金:Agriculture and Food Research Initiative Competitive Grant no.2018-67015-31936 from the USDA National Institute of Food and Agriculture.
摘 要:Background MicroRNAs(miRNAs)are presented in the uterine lumen of many mammals,and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast functions.Our aim was to identify and contrast the miRNAs present in extracellular vesicles(EVs)in the uterine lumen fluid(ULF)at the onset of attachment in cattle pregnancies(gestation d 18)initiated by artificial insemination(AI)or by the trans-fer of an in vitro-produced blastocyst(IVP-ET).A third group had no conceptus after the transfer of an IVP embryo.Results The abundance of 263 annotated miRNAs was quantified in the EVs collected from ULF.There was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group,while 4 miRNAs had a lower abundance relative to the group not containing a conceptus.Additionally,4 miRNAs were more abun-dant in ULF EVs in the AI pregnant group relative to IVP-ET group(bta-mir-17,bta-mir-7-3,MIR7-1,MIR18A).Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endome-trium,including genes that are known to be their targets.Conclusions The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation,as well as in endometrium receptivity.The knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of cor-rective approaches to reduce conceptus losses during the first month of pregnancy in cattle.
关 键 词:Artificial reproductive technology CATTLE CONCEPTUS Embryos ENDOMETRIUM Small RNA
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.28