Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid  

作　　者：Jean M.Feugang Ahmed Gad Nico G.Menjivar Ghassan M.Ishak Samuel Gebremedhn Melba O.Gastal Notsile H.Dlamini Radek Prochazka Eduardo L.Gastal Dawit Tesfaye 

机构地区：[1]Department of Animal and Dairy Sciences,Mississippi State University,Mississippi State,MS 39762,USA [2]Animal Reproduction and Biotechnology Laboratory(ARBL),Department of Biomedical Sciences,College of Veterinary Medicine and Biomedical Sciences,Colorado State University,Fort Collins,CO 80523,USA [3]Department of Animal Production,Faculty of Agriculture,Cairo University,Giza 12613,Egypt [4]Department of Surgery and Obstetrics,College of Veterinary Medicine,University of Baghdad,Baghdad 10011,Iraq [5]Animal Science,School of Agricultural Sciences,Southern Illinois University,Carbondale,IL 62901,USA [6]J.R.Simplot Company,Kuna,ID 83634,USA [7]Institute of Animal Physiology and Genetics,Czech Academy of Sciences,Liběchov 27721,Czech Republic

出　　处：《Journal of Animal Science and Biotechnology》2025年第1期136-152,共17页畜牧与生物技术杂志(英文版)

基　　金：Southern Illinois University,Carbondale,IL;Ministry of Higher Education&Scientific Research,Baghdad,Iraq;NIFA-USDA Hatch project accession#1016077(Multistate#W4171);USDAARS project 6066-31000-015-00D;NIH MS-IDeA network of Biomedical Research Excellence award 5P20GMI03476-19.GMI received a PhD scholarship from the Ministry of Higher Education&Scientific Research,Baghdad,Iraq.

摘　　要：Background Ovarian follicular fluid(FF)is a dynamic environment that changes with the seasons,affecting follicle development,ovulation,and oocyte quality.Cells in the follicles release tiny particles called extracellular vesicles(EVs)containing vital regulatory molecules,such as microRNAs(miRNAs).These miRNAs are pivotal in facilitating commu-nication within the follicles through diverse signaling and information transfer forms.EV-coupled miRNA signaling is implicated to be associated with ovarian function,follicle and oocyte growth and response to various environmen-tal insults.Herein,we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in horses.Results Ultrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the ano-vulatory(spring:non-breeding)and ovulatory(spring,summer,and fall:breeding)seasons and subsequent EV isola-tion and miRNA profiling identified significant variation in EV-miRNA cargo content.We identified 97 miRNAs with dif-ferential expression among the groups and specific clusters of miRNAs involved in the spring transition(miR-149,-200b,-206,-221,-328,and-615)and peak breeding period(including miR-143,-192,-451,-302b,-100,and let-7c).Bioinformatic analyses showed enrichments in various biological functions,e.g.,transcription factor activity,transcrip-tion and transcription regulation,nucleic acid binding,sequence-specific DNA binding,p53 signaling,and post-trans-lational modifications.Cluster analyses revealed distinct sets of significantly up-and down-regulated miRNAs associ-ated with spring anovulatory(Cluster 1)and summer ovulation–the peak breeding season(Clusters 4 and 6).Conclusions The findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons,and their roles in understanding the mechanisms involved in seasonal shifts and ovu

关 键 词：Extracellular vesicle Follicle growth Follicular fluid HORSE MARE OVULATION Pre-ovulation Seasonal breeding 

分 类 号：R73[医药卫生—肿瘤]