GSTT1、GSTM1和EPHX1基因对煤焦油沥青作业工人皮肤损伤的影响  

Effects of GSTT1,GSTM1 and EPHX1 genes on skin injury in workers exposed to coal tar pitch

作　　者：孙晓晨 李宁[1] 宋丰江 苏东红 王韬 张放[2] 崔峰[1] 邵华[2] SUN Xiaochen;LI Ning;SONG Fengjiang;SU Donghong;WANG Tao;ZHANG Fang;CUI Feng;SHAO Hua(Zibo Center for Disease Control and Prevention,Zibo,Shandong 255000,China)

机构地区：[1]淄博市疾病预防控制中心,山东淄博255000 [2]山东省医学科学院山东省职业卫生与职业病防治研究院,山东济南250000

出　　处：《中国职业医学》2024年第5期528-532,共5页China Occupational Medicine

基　　金：山东省中医药科技项目(Z-2022083);淄博市医药卫生科研项目青年项目(20241701100)。

摘　　要：目的探讨谷胱甘肽硫转移酶(GST)T1、GSTM1基因和环氧化物水解酶(EPHX1)基因对煤焦油沥青职业接触工人皮肤损伤的影响。方法采用判断抽样方法,选择某碳素企业中煤焦油沥青生产和使用企业的作业工人为研究对象,以其中接触煤焦油沥青后出现皮肤损伤者为病例组(55例),以同车间同工种且未出现皮肤异常者为对照组(197例)。采集研究对象尿样和血样,采用超高效液相色谱串联质谱仪测定尿中多环芳烃代谢产物1-羟基芘(1-OH-P)、1-羟基萘(1-OH-N)和2-羟基萘(2-OH-N)水平;采用聚合酶链式反应技术检测血中GSTT1、GSTM1和EPHX1基因。结果55例病例组作业工人均出现皮肤刺痛,25例出现皮肤瘙痒和脱屑,各有15例出现黑头粉刺和色素沉着。病例组人群尿中1-OH-N和2-OH-N水平均低于对照组(P值均<0.05);但2组人群尿中1-OH-P水平比较,差异无统计学意义(P>0.05)。2组人群血中GSTT1、GSTM1和EPHX1(His139His)基因检出人数比较,差异均有统计学意义(P值均<0.01)。携带GSTT1和GSTM1基因分别与班后尿中1-OH-N、1-OH-P和2-OH-N水平呈正相关关系(P值均<0.01);而携带EPHX1(139Arg位点)基因仅与班后尿中的2-OH-N呈正相关关系(P=0.03)。在排除年龄、工龄、性别、吸烟和饮酒的影响后,携带GSTT1、GSTM1和EPHX1(139Arg位点)基因可减轻煤焦油沥青作业工人的皮肤损伤(P值均<0.01)。结论接触煤焦油沥青可造成作业工人出现皮肤损伤;携带GSTT1、GSTM1和EPHX1(139Arg位点)基因是煤焦油沥青作业工人皮肤损伤的保护因素。Objective To investigate the effects of glutathiones-transferase(GST)T1,GSTM1 and epoxide hydrolase(EPHX1)genes on skin injury in workers exposed to coal tar pitch.Methods Workers from a carbon manufacturing company involved in coal tar pitch production and use were selected as the study subjects using a judgment sampling method.Workers with skin injury after exposed to coal tar were selected as the case group(55 cases),and those with the same workshop and type of work but without skin abnormalities were selected as the control group(197 cases).Urine and blood samples were collected from the workers,and levels of polycyclic aromatic hydrocarbon metabolites,including 1-pyrenol(1-OH-P),1-naphthol(1-OH-N)and 2-naphthol(2-OH-N),in urine were measured using ultra high-performance liquid chromatography tandem mass spectrometry.The GSTT1,GSTM1 and EPHX1 genes in blood were detected by polymerase chain reaction.Results In the case group,all 55 workers reported skin stinging,25 workers reported itching and flaking,and 15 workers reported blackheads and pigmentation.Urinary levels of 1-OH-N and 2-OH-N were lower in the worker in the case group than that in the control group(all P<0.05).However,there was no significant difference in the level of 1-OH-P between the two groups(P>0.05).There were significant differences in the number of workers with GSTT1,GSTM1 and EPHX1(His139His)genes between the two groups(all P<0.01).The GSTT1 and GSTM1 genes were positively correlated with post-shift urinary levels of 1-OH-N,1-OH-P,and 2-OH-N(all P<0.01).The EPHX1(139Arg locus)gene was positively correlated with post-shift 2-OH-N levels(P=0.03).The GSTT1,GSTM1,and EPHX1(139Arg locus)genes were associated with reduced skin damage among coal tar workers(all P<0.01),after controlling for age,length of service,gender,smoking,and alcohol consumption.Conclusion Exposure to coal tar pitch can cause skin injury in workers,and the GSTT1,GSTM1,and EPHX1(139Arg locus)genes are protective factors against skin injury in those workers.

关 键 词：基因 皮肤损伤 煤焦油沥青 职业接触 

分 类 号：R135.7[医药卫生—劳动卫生]