湖北省3例婴幼儿病例中人肠道致细胞病变孤儿病毒30型的遗传进化分析  

Genetic evolution analysis of enteric cytopathogenic human orphan virus type 30 in three cases of infants and young children in Hubei Province

作　　者：周康平 胡兵[1] 潘锴 蔡昆[1] Zhou Kangping;Hu Bing;Pan Kai;Cai Kun(Health Inspection and Testing Institute,Hubei Provincial Center for Disease Control and Prevention,Wuhan430079,China)

机构地区：[1]湖北省疾病预防控制中心卫生检验检测研究所,武汉430079

出　　处：《中华预防医学杂志》2025年第1期95-100,共6页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金项目(U21A20423);病毒学国家重点实验室开放研究基金(2022KF001);湖北省公共卫生青年拔尖人才项目;楚天英才医疗卫生人才项目。

摘　　要：湖北省3例婴幼儿病例的咽拭子样本经鉴定均为肠道病毒;年龄均为1月龄;发病时间均为6月龄,均为散发型病例,无明确流行病学关联。VP1基因扩增分析结果显示,3例婴幼儿病例均感染了埃可病毒30型(ECHO30),3例样本分离得到的ECHO30湖北株均归类于H基因亚型的ClusterⅢ簇。全基因组扩增分析结果显示,ECHO30湖北株与GenBank中的ECHO30/MW586892/Ashburton/17WQ2027G/USA/2017-08-20同源性最高,核苷酸与氨基酸同源性分别为97.6%和99.3%。重组分析结果显示,ECHO30湖北株在非结构蛋白区存在重组,重组片段可能来自ECHO30/KY888274/14-397/Germany/2013和ECHO18株Echo18/MN815811/BJ2018-S6363/Beijing/2018。本研究对湖北省流行的ECHO30毒株遗传进化进行了深入分析,能够为预防ECHO30感染提供基础数据。Three throat swab samples from infants and young children in Hubei Province were identified as positive for enteroviruses.All subjects were one month old,and the onset of symptoms occurred in June;these cases were sporadic and exhibited no clear epidemiological associations.The results of VP1 gene amplification analysis indicated that all three infant cases were attributed to Echovirus 30(ECHO30).The ECHO30 strain isolated from the three samples was classified as ClusterⅢwithin the H gene subtype.Whole genome amplification analysis revealed that the ECHO30 strain from Hubei exhibited the highest homology with ECHO30/MW586892/Ashburton/17WQ2027G/USA/2007-08-20 from GenBank,demonstrating nucleotide and amino acid homologies of 97.6%and 99.3%,respectively.Recombination analysis indicated the presence of recombination events in the non-structural protein region of the ECHO30 Hubei strain,with recombinant fragments potentially originating from ECHO30/KY888274/14-397/Germany/2013 and ECHO18 strains Echo18/MN815811/BJ2018-S6363/Beijing/2018.This study provides a comprehensive analysis of the genetic evolution of the ECHO30 strain prevalent in Hubei Province,contributing essential data for the prevention of ECHO30 infections.

关 键 词：埃可病毒30型 全基因组 基因进化分析 基因重组分析 

分 类 号：R725.1[医药卫生—儿科]