甜瓜抗霜霉病KASP分子标记的开发与验证  

Development and verification of KASP marker for resistance to downy mildew in muskmelon

作　　者：沈悦 凌悦铭 段晓宇 杨文莉 李寐华[2] 王懿柔 王惠林[1] 张学军[2] SHEN Yue;LING Yueming;DUAN Xiaoyu;YANG Wenli;LI Meihua;WANG Yirou;WANG Huilin;ZHANG Xuejun(College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China;Hami Melon Research Center,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学园艺学院,乌鲁木齐830052 [2]新疆农业科学院哈密瓜研究中心,乌鲁木齐830091

出　　处：《新疆农业科学》2024年第11期2626-2634,共9页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区天山创新团队(2022D14015);国家自然科学基金项目(32060689);财政部和农业农村部“国家现代农业产业技术体系资助”项目(CARS-25);海南省院士创新平台科研专项(吴明珠院士团队创新中心);海南省院士创新平台科研(YSPTZX202141);新疆维吾尔自治区重点研发任务专项(2022B02002)。

摘　　要：【目的】开发及验证与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记,为甜瓜抗霜霉病分子标记辅助育种提供理论基础。【方法】以高抗霜霉病甜瓜品种“PI 438685”与高感霜霉病甜瓜品种“黄旦子”为亲本,构建F 2代分离群体。利用BSA-seq技术,对甜瓜霜霉病抗性基因进行QTL定位,根据SNP分布位置在定位区间内开发66对与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记,并使用F 2代分离群体进行筛选验证。【结果】霜霉病抗性基因定位到9号染色体末端区域,筛选出9对在双亲间具有多态性的KASP分子标记。获得1对与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记(KASP_24832572),其基因分型准确率为79%,准确度较高。【结论】在9号染色体上定位到1个与甜瓜霜霉病抗性相关的区间,在此区间内开发并筛选出1对与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记,该标记有助于甜瓜抗霜霉病分子标记辅助育种。【Objective】This study aims to develop and validate KASP molecular markers closely linked to melon downy mildew resistance genes,in the hope of providing a theoretical basis for downy mildew resistance molecular marker assisted breeding of downy mildew resistance in melons.【Methods】The accession‘PI 438685’with high resistance of downy mildew,and the accession‘Huangdanzi’with high sensitivity of downy mildew were used as parents to construct a separate population.QTL mapping of downy mildew resistance genes in melon was drawn through a combination of BSA and whole genome sequencing.KASP molecular markers closely linked to downy mildew resistance genes were developed within this interval,and then screened and validated using F 2 separate populations.【Results】The downy mildew resistance gene was located on chromosome 9 through BSA analysis.We developed 66 pair of KASP molecular markers in the candidate region,screened 9 pairs of KASP molecular markers with high specificity.Subsequently,the 9 pairs of KASP molecular markers were validated,the KASP molecular marker(KASP-24832572),closely linked to downy mildew resistance genes was obtained,and its accuracy of distinguish genotype could reach 79%.【Conclusion】One QTL related to downy mildew resistance has been located on chromosome 9 by BSA analysis,and a pair of KASP molecular markers closely linked to downy mildew resistance genes are developed in this interval,which has contributed to downy mildew resistance breeding in melons.

关 键 词：甜瓜 霜霉病 BSA-seq KASP分子标记 分子标记辅助育种 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]