基于核磁共振代谢组学揭示黄精通过调控苯丙氨酸、酪氨酸及色氨酸的生物合成发挥抗疲劳作用机制  

Anti-fatigue effect of Huangjing by regulating the biosynthesis of phenylalanine,tyrosine and tryptophan based on nuclear magnetic resonance metabolomics

作　　者：陈磊 杨莹莹 连让平 但春凤 刘海江 李爱平[3,4] 杨文彬 Chen Lei;Yang Yingying;Lian Rangping;Dan Chunfeng;Liu Haijiang;Li Aiping;Yang Wenbin(Shanxi Provincial Drug Evaluation Center/Shanxi Institute of Medicine and Life Sciences,Taiyuan Shanxi 030006;Center for Drug Inspection of Shanxi Province/Center for Vaccine Inspection of Shanxi Province,Taiyuan Shanxi 030031;Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan Shanxi 030006;The Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province,Taiyuan Shanxi 030006)

机构地区：[1]山西省药品审评中心/山西省医药与生命科学研究院,山西太原030006 [2]山西省药品检查中心/山西省疫苗检查中心,山西太原030031 [3]山西大学中医药现代研究中心,山西太原030006 [4]地产中药功效物质研究与利用山西省重点实验室,山西太原030006

出　　处：《山西中医药大学学报》2025年第1期27-34,共8页Journal of Shanxi University of Chinese Medicine

基　　金：山西省重点研发计划资助项目(201903D321221);山西省面上青年基金资助项目(201901D211573)。

摘　　要：目的:运用核磁共振(1H NMR)代谢组学方法,研究黄精的抗疲劳作用及可能的作用机制。方法:30只雄性ICR小鼠随机分为对照组、黄精低、高剂量组,每组10只。低、高剂量组分别灌胃给予剂量为1.125 g/kg、2.5 g/kg的黄精水提物,对照组给予相同体积蒸馏水,灌胃1次/d,连续3周。灌胃结束后进行负重力竭游泳实验,小鼠达到疲劳状态后,测试体质量变化肝脏肝糖原含量、血清乳酸及尿素氮(BUN)水平。进一步采用1H NMR代谢组学技术寻找小鼠腓肠肌内源性代谢物的变化,并进行接收者操作特征曲线(ROC)分析,筛选ROC曲线下面积>0.7的差异代谢物输入MetaboAnalyst网站中进行通路富集分析。结果:3组小鼠体质量均增加,但差异无统计学意义(P>0.05);与对照组比较,黄精高、低剂量组小鼠力竭游泳时间提高,肝糖原含量明显增高,血乳酸含量明显下降,差异均有统计学意义(P<0.01,P<0.001);黄精高、低剂量组小鼠血清BUN水平均下降,但差异无统计学意义(P>0.05)。在小鼠腓肠肌组织提取物中共指认34种代谢物,其中21个差异代谢物在黄精干预后发生显著变化,结合ROC曲线,筛选出17个差异代谢物。通路分析发现,黄精主要通过调控苯丙氨酸、酪氨酸及色氨酸的生物合成途径来发挥抗疲劳作用。结论:本研究采用1H NMR代谢组学技术结合多元统计分析阐明黄精的抗疲劳作用机制,为黄精抗疲劳应用以及相关产品研发提供理论依据。Objective:To investigate the anti-fatigue effect of Huangjing and its possible mechanism based on nuclear magnetic resonance(1H NMR)metabolomics.Methods:30 male ICR mice were randomly divided into control group,lowdose and high-dose of Huangjing groups,with 10 mice in each group.The mice in the low-dose and high-dose groups were given intragastric administration of water extract of Huangjing at doses of 1.125 g/kg and 2.5 g/kg,respectively,while the mice in the control group were given the same volume of distilled water.Gavage was given once a day for 3 consecutive weeks.After the end of gavage,exhaustive weight-bearing swimming test was performed.After the mice reach a state of fatigue,changes in body mess and liver glycogen content,serum lactic acid and blood urea nitrogen(BUN)levels were measured.Furthermore,the 1H NMR metabolomics technology was used to find the changes of endogenous metabolites in the gastrocnemius muscle of mice,and the receiver operating characteristic curve(ROC)analysis was performed.The differential metabolites with area under the ROC curve>0.7 were selected and entered into the MetaboAnalyst website for pathway enrichment analysis.Results:The body mass of mice in three groups was increased,but the difference was not statistically significant(P>0.05).Compared with that of the control group,the mice in the high/low dose of Huangjing group had a significantly increased exhaustive swimming time,a significantly increased liver glycogen content,and a significantly decreased blood lactic acid content,with statistical significance(P<0.01,P<0.001).The levels of BUN in the high and low dose of Huangjing groups decreased,but the difference was not statistically significant(P>0.05).34 metabolites were identified in mice gastrocnemius muscle tissue extracts,of which 21 differential metabolites were significantly changed after the Huangjing intervention.Combined with ROC curve,17 differential metabolites were screened out.Pathway analysis showed that Huangjing exerted its anti-fatigue effect mainly b

关 键 词：黄精 抗疲劳 腓肠肌 代谢组学 苯丙氨酸、酪氨酸及色氨酸的生物合成 

分 类 号：R285.5[医药卫生—中药学]