黄芪皂苷Ⅱ成分对三阴性乳腺癌细胞增殖及侵袭转移的抑制作用研究  

Inhibitory Effect of Astragaloside Ⅱ on Cell Proliferation,Invasion and Metastasis in Triple-negative Breast Cancer

作　　者：殷生楠[1] 高一 戴怀洋 王刚 殷勇[1] YIN Sheng-nan;GAO Yi;DAI Huai-yang;WANG Gang;YIN Yong(Taizhou Hospital of Traditional Chinese Medicine;Taizhou Fourth People’s Hospital,Taizhou Jiangsu 225300;Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712000,China)

机构地区：[1]泰州市中医院 [2]泰州市第四人民医院,江苏泰州225300 [3]陕西中医药大学药学院,陕西咸阳712000

出　　处：《泰州职业技术学院学报》2025年第1期63-66,96,共5页Journal of Taizhou Polytechnic College

基　　金：泰州市中医药科技发展项目(TZ202113,项目主持人:殷生楠);泰州市科技支撑计划(社发)指导性项目(202103,项目主持人:殷勇).

摘　　要：目的探究三萜皂苷黄芪皂苷Ⅱ(AstragalosideⅡ,ASⅡ)对三阴乳腺癌(Triple nega⁃tive breast cancer,TNBC)细胞增殖及侵袭转移的作用及可能的机制。方法采用MTT法检测黄芪皂苷Ⅱ(0.78μM、1.56μM、3.12μM、6.25μM、12.5μM、25μM、50μM、100μM)对MDA-MB-231细胞增殖的影响;采用Transwell法及划痕实验分别检测黄芪皂苷Ⅱ对MDA-MB-231细胞转移及侵袭的影响;采用Western blot检测PI3K/AKT/mTOR通路蛋白的表达水平,从而阐明其调控PI3K/AKT/mTOR通路的分子机制。结果25μM黄芪皂苷Ⅱ显著抑制MDA-MB-231细胞增殖,选用未影响细胞增殖的药物浓度3.12μM、6.25μM、12.5μM作为低、中、高药物浓度;与对照组相比,黄芪皂苷Ⅱ呈浓度依赖性地抑制MDA-MB-231细胞的侵袭与转移;同时,该成分抑制MDA-MB-231细胞PI3K、AKT、mTOR蛋白磷酸化水平。结论黄芪皂苷Ⅱ可能通过调控PI3K/AKT/mTOR通路抑制MDA-MB-231细胞增殖、侵袭及转移。Objective To explore the effect and possible mechanism of AstragalosideⅡon the proliferation,invasion and metastasis of triple negative breast cancer cells.Methods Cell proliferation assay was used to test the effects of Astragaloside Ⅱ (0.78μM,1.56μM,3.12μM,6.25μM,2.5μM,12.5μM,25μM,50μM,100μM)on MDA-MB-231 cell proliferation.The effect of Astragaloside Ⅱ on MDA-MB-231 cells were detected by Transwell method and scratch assay.The expression levels of proteins in the PI3K/AKT/mTOR pathway were determined by Western blot,thus elucidating the molecular mechanism of PI3K/AKT/mTOR pathway.Results The results showed that Astragaloside Ⅱ concentration of 25μM significantly inhibited MDA-MB-231 cell proliferation,drug concentrations of 3.12μM,6.25μM,and 12.5μM that did not affect proliferation were selected as the low,medium,and high drug concentrations;Compared with the control group,Astragaloside Ⅱ concentration-dependently inhibited the invasion and metastasis of MDA-MB-231 cells and the phosphorylation levels of PI3K,AKT,and mTOR proteins in MDA-MB-231 cells.Conclusion Astragaloside Ⅱ inhibited the proliferation,invasion and metastasis of MDA-MB-231 cells by acting on the PI3K/AKT/mTOR pathway.

关 键 词：黄芪皂苷Ⅱ 三阴性乳腺癌 转移 PI3K/AKT/mTOR通路 

分 类 号：R285.5[医药卫生—中药学] R737.9[医药卫生—中医学]