丹参转录因子及相关调控蛋白研究进展  

Research progress on transcription factors and regulatory proteins of Salvia miltiorrhiza

作　　者：徐文 田梅 申业[1] 郭娟 靳保龙 崔光红[1] XU Wen;TIAN Mei;SHEN Ye;GUO Juan;JIN Bao-long;CUI Guang-hong(State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室,北京100700

出　　处：《中国中药杂志》2025年第1期58-70,共13页China Journal of Chinese Materia Medica

基　　金：中国中医科学院科技创新工程项目(CI2021A04109);国家自然科学基金项目(82274054)。

摘　　要：丹参Salvia miltiorrhiza为唇形科鼠尾草属植物,为最早进行分子生物学研究的药用植物之一,并逐渐成为药用植物分子生物学研究的模式植物。随着丹参基因组及其主要活性成分丹参酮、丹酚酸类化合物生物合成途径的逐步解析,由转录因子及相关调控蛋白介导的转录调控研究逐步成为新的热点。由于不同文献对转录因子缺乏科学一致的命名,研究指标各不相同等问题,该文使用自交3代紫花丹参DSS3和自交6代白花丹参bh2-7的基因组作为参考,对不同文献中的转录因子进行了系统的整理,确定了来源于13个基因家族,共计73个丹参转录因子及相关调控蛋白的功能。同时也对相关基因过表达或基因沉默实验后对丹参酮和丹酚酸类成分的影响进行了统计。这些工作从源头统一了已鉴定的转录因子,为后续深入开展丹参中次生代谢产物及抗虫、抗逆等调控网络的构建和利用全局转录调控工程提高药材品质研究奠定了基础。Salvia miltiorrhiza is a perennial herb of the genus Salvia(Lamiaceae).As one of the earliest medicinal plants to undergo molecular biology research,it has gradually become a model plant for molecular biology of medicinal plants.With the gradual analysis of the genome of S.miltiorrhiza and the biosynthetic pathways of its main active components tanshinone and salvianolic acids,the transcriptional regulation mediated by transcription factors and related regulatory proteins has gradually become a new research focus.Due to the lack of scientific and unified naming of transcription factors and different research indexes in different literature,this paper systematically sorted out the transcription factors in different literature with the genomes of DSS3 from selfing for three generations and bh2-7 from selfing for six generations as reference.In total,73 transcription factors and related regulatory proteins belonging to 13 gene families were identified.The effects of overexpression or gene silencing experiments on tanshinone and salvianolic acids were also analyzed.This study unified the identified transcription factors,which laid a foundation for further constructing the regulatory networks of secondary metabolites and insect or stress resistance and improving the quality of medicinal materials by using global transcriptional regulation engineering.

关 键 词：丹参 转录因子 调控蛋白 

分 类 号：S567.53[农业科学—中草药栽培]