基于指纹图谱和含量测定结合化学计量学发现川芎道地性标志物  

Comprehensive application of fingerprint studies,content determination,and chemometrics to identify geo-markers of Chuanxiong Rhizoma

作　　者：武梦园 彭成[1] 蒙春旺 刘娟汝 周勤梅[1] 戴鸥[1] 熊亮[1,2] WU Meng-yuan;PENG Cheng;MENG Chun-wang;LIU Juan-ru;ZHOU Qin-mei;DAI Ou;XIONG Liang(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;College of Medical Technology,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院/现代中药产业学院西南特色中药资源国家重点实验室,四川成都611137 [2]成都中医药大学医学技术学院,四川成都611137

出　　处：《中国中药杂志》2025年第1期152-164,共13页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(82374152);四川省科技创新创业苗子工程项目(2023JDRC0122);四川省科技创新人才计划项目(2023JDRC0041)。

摘　　要：该研究建立不同产地川芎样品的指纹图谱,并结合化学计量学方法筛选川芎潜在的产地差异性成分,进一步采用含量测定法表征上述差异性成分在各产地药材中的含量差异,再结合化学计量学方法发现可用于区分道地川芎和非道地川芎的道地性标志物。在指纹图谱研究中,共标定27个共有峰,37批不同产地川芎指纹图谱的相似度均在0.968以上;采用正交偏最小二乘法判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)可区分四川与其他3个省区川芎,以及四川道地(都江堰)川芎与四川非道地川芎,且通过OPLS-DA项下的变量重要度投影(variable importance in projection,VIP)分析筛选出不同省区(14个)和四川省内(16个)的潜在产地差异性成分。采用对照品指认出共有峰中10个潜在的产地差异性成分,随后经含量测定证实10个潜在产地差异性成分在不同产地间存在含量差异。在此基础上,以产地差异性成分的含量为变量,进行OPLS-DA及VIP分析,明确了Z-藁本内酯、绿原酸和丁烯基苯酞/洋川芎内酯A比值是区分四川川芎与陕西、河北、江西川芎的道地性标志物;Z-藁本内酯、正丁基苯酞、Z-藁本内酯/洋川芎内酯A比值和丁烯基苯酞/洋川芎内酯A比值是区分道地(都江堰)川芎和四川非道地川芎的道地性标志物。该文基于指纹图谱和含量测定结合化学计量学初步阐释了道地与非道地川芎的物质基础差异,为道地中药材的物质基础研究提供了一定的参考。This study established a high performance liquid chromatography(HPLC)fingerprint of Chuanxiong Rhizoma from different producing areas and screened its potential differential components for producing areas by chemometrics.Furthermore,the content of the above differential components in Chuanxiong Rhizoma from different producing areas was measured and compared.Then,the geoherbalism markers(geo-markers)that can be used to distinguish Dao-di and non-Dao-di Chuanxiong Rhizoma were excavated by chemometrics.In fingerprint studies,a total of 27 common peaks were determined,and the fingerprint similarity for 37 batches of Chuanxiong Rhizoma samples from different producing areas was above 0.968.The orthogonal partial least squares-discriminant analysis(OPLS-DA)was capable of distinguishing Chuanxiong Rhizoma from Sichuan and from three other provinces,as well as Dao-di Chuanxiong Rhizoma(from Dujiangyan)and non-Dao-di Chuanxiong Rhizoma(from other producing areas)in Sichuan province.Meanwhile,14 potential differential components in Chuanxiong Rhizoma from different provinces and 16 potential differential components in Chuanxiong Rhizoma from different producing areas in Sichuan were screened by the variable importance in projection(VIP)analysis under OPLS-DA.The reference standards were used to identify 10 potential differential components in the common peaks,and subsequent content determination verified that the content of the above 10 potential differential components was different among different producing areas.Then,the OPLS-DA and VIP analysis were performed with the content of the 10 potential differential components as variables.The results showed that Z-ligustilide,chlorogenic acid,and the ratio of butylidenephthalide/senkyunolide A were the geo-markers that can distinguish Chuanxiong Rhizoma from Sichuan and Chuanxiong Rhizoma from Shaanxi,Hebei,and Jiangxi,while Z-ligustilide,n-butylphthalide,and the ratios of Z-ligustilide/senkyunolide A and butylidenephthalide/senkyunolide A were the geo-markers that can dist

关 键 词：川芎 指纹图谱 含量测定 化学计量学 道地性标志物 

分 类 号：R284.1[医药卫生—中药学]