Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens  

作　　者：Minghui Wang Kelin Li Hongchao Jiao Jingpeng Zhao Haifang Li Yunlei Zhou Aizhi Cao Jianmin Wang Xiaojuan Wang Hai Lin 

机构地区：[1]College of Animal Science and Technology,Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Key Laboratory of Efficient Utilization of Non‑Grain Feed Resources(Co‑Construction By Ministry and Province),Ministry of Agriculture and Rural Affairs,Shandong Agricultural University,No.61,Daizong Street,Taian 271018,Shandong,P.R.China [2]College of Life Sciences,Shandong Agricultural University,No.61,Daizong Street,Taian 271018,Shandong,P.R.China [3]College of Chemistry and Material Science,Shandong Agricultural University,No.61,Daizong Street,Taian 271018,Shandong,P.R.China [4]Shandong Longchang Animal Health Products Co.,Ltd.,Jinan,P.R.China

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2483-2505,共23页畜牧与生物技术杂志(英文版)

基　　金：supported by grants from Key Projects of the National Natural Science Foundation of China(32330101);Natural Science Foundation of Shandong Province(ZR2023QC002);Key Technology Research and Development Program of Shandong province(2019JZZY020602);the Earmarked Fund for China Agriculture Research System(CARS-40-K09)。

摘　　要：Background High-fat diets(HFD)are known to enhance feed conversion ratio in broiler chickens,yet they can also result in hepatic fat accumulation.Bile acids(BAs)and gut microbiota also play key roles in the formation of fatty liver.In this study,our objective was to elucidate the mechanisms through which BA supplementation reduces hepatic fat deposition in broiler chickens,with a focus on the involvement of gut microbiota and liver BA composition.Results Newly hatched broiler chickens were allocated to either a low-fat diet(LFD)or HFD,supplemented with or without BAs,and subsequently assessed their impacts on gut microbiota,hepatic lipid metabolism,and hepatic BA composition.Our findings showed that BA supplementation significantly reduced plasma and liver tissue triglyceride(TG)levels in 42-dav-old broiler chickens(P<0.05),concurrently with a significant decrease in the expression levels of fatty acid synthase(FAS)in liver tissue(P<0.05).These results suggest that BA supplementation effectively diminishes hepatic fat deposition.Under the LFD,BAs supplementation increased the BA content and ratio of Non 12-OH BAs/12-OH BAs in the liver and increased the Akkermansia abundance in cecum.Under the HFD,BA supplementation decreased the BAs and increased the relative abundances of chenodeoxycholic acid(CDCA)and cholic acid(CA)in hepatic tissue,while the relative abundances of Bacteroides were dramatically reduced and the Bifidobacterium,Escherichia,and Lactobacillus were increased in cecum.Correlation analyses showed a significant positive correlation between the Akkermansia abundance and Non 12-OH BA content under the LFD,and presented a significant negative correlation between the Bacteroides abundance and CA or CDCA content under the HFD.Conclusions The results indicate that supplementation of BAs in both LFD and HFD may ameliorate hepatic fat deposition in broiler chickens with the involvement of differentiated microbiota-bile acid profile pathways.

关 键 词：Bile acids Broiler chickens Gut microbiota Hepatic fat deposition Liver bile acid profile 

分 类 号：S831.5[农业科学—畜牧学]