Dietary silymarin improves performance by altering hepatic lipid metabolism and cecal microbiota function and its metabolites in late laying hens  

作　　者：Yanghao Guo Yudong Xu Derun Wang Shihao Yang Zehe Song Rui Li Xi He 

机构地区：[1]College of Animal Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China [2]Hunan Engineering Research Center of Poultry Production Safety,Changsha,Hunan 410128,China [3]Yuelushan Laboratory,Hunan Agricultural University,Changsha,Hunan 410128,China [4]Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2553-2570,共18页畜牧与生物技术杂志(英文版)

基　　金：National Key R&D Program of China:2023YFD1301200;Hunan Poultry Industry Technology System for providing fund support。

摘　　要：Background Liver lipid dysregulation is one of the major factors in the decline of production performance in latestage laying hens.Silymarin(SIL),a natural flavonolignan extracted from milk thistle,is known for its hepatoprotective and lipid-lowering properties in humans.This study evaluates whether SIL can provide similar benefits to late-stage laying hens.A total of 48068-week-old Lohmann Pink laying hens were randomly assigned into 5 groups,each group consisting of 6 replicates with 16 hens each.The birds received a basal diet either without silymarin(control)or supplemented with silymarin at concentrations of 250,500,750,or 1,000 mg/kg(SIL250,SIL500,SIL750,SIL1000)over a 12-week period.Results The CON group exhibited a significant decline in laying rates from weeks 9 to 12 compared to the initial4 weeks(P=0.042),while SIL supplementation maintained consistent laying rates throughout the study(P>0.05).Notably,the SIL500 and SIL750 groups showed higher average egg weight than the CON group during weeks 5 to 8(P=0.049).The SIL750 group had a significantly higher average daily feed intake across the study period(P<0.05),and the SIL500 group saw a marked decrease in the feed-to-egg ratio from weeks 5 to 8(P=0.003).Furthermore,the SIL500 group demonstrated significant reductions in serum ALT and AST levels(P<0.05)and a significant decrease in serum triglycerides and total cholesterol at week 12 with increasing doses of SIL(P<0.05).SIL also positively influenced liver enzyme expression(FASN,ACC,Apo-VLDLⅡ,FXR,and CYP7A1;P<0.05)and altered the cecal microbiota composition,enhancing species linked to secondary bile acid synthesis.Targeted metabolomics identified 9 metabolites predominantly involved in thiamin metabolism that were significantly different in the SIL groups(P<0.05).Conclusions Our study demonstrated that dietary SIL supplementation could ameliorate egg production rate in late stage laying hens,mechanistically,this effect was via improving hepatic lipid metabolism and cecal microbiota function to achieve

关 键 词：Bile acid metabolism Cecal microbiota Laying hen Lipid metabolism LIPOPROTEINS SILYMARIN 

分 类 号：S831.5[农业科学—畜牧学]