Hepatoprotective effects of magnolol in fatty liver hemorrhagic syndrome hens through shaping gut microbiota and tryptophan metabolic profil  

作　　者：Yujie Lv Chaoyue Ge Lianchi Wu Zhaoying Hu Xinyu Luo Weichen Huang Shenao Zhan Xinyu Shen Dongyou Yu Bing Liu 

机构地区：[1]Hainan Institute,Zhejiang University,572000 Sanya,China [2]College of Animal Sciences,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2589-2606,共18页畜牧与生物技术杂志(英文版)

基　　金：financially supported by the Key Research&Development Program of Zhejiang Province(Grant no:2024C02004);the Collaborative Extension Program for Advancements in Agricultural Technologies of Zhejiang Province(Grant no:2023ZDXT15)。

摘　　要：Background Magnolol(MAG)exhibits hepatoprotective activity,however,whether and how MAG regulates the gut microbiota to alleviate fatty liv er hemorrhagic syndrome(FLH S)remains unclear.Therefore,we investigated the mechanism of MAG in FLHS laying hens with an emphasis on alterations in the gut-liver axis.We randomly divided 54056-week-old Hy-line white laying hens with FLSH into 4 groups.The birds were fed a high-fat low-protein(HFLP)diet(CON).or HELP diets supplemented with 200,400,and 600 mg/kg of MAG(M1,M2,and M3,respectively)for 9 weeks.Results Magnolol supplementation increased the laying rate and ameliorated hepatic damage and dysfunction by regulating lipid metabolism,improving intestinal barrier function,and shaping the gut microbiota and tryptophan metabolic profiles.Dietary MAG supplementation downregulated the expression of lipid synthesis genes and upregulated the expression of lipid transport genes at varying degrees.The intestinal barrier function was improved by 200and 400 mg/kg of MAG supplementation,as evidenced by the increased villus height and mRNA expression of tight junction related genes.Microbiological profile information revealed that MAG changed the gut microbiota,especially by elevating the abundances of Lactobacillus,Faecallbacterium,and Butyricicoccus.Moreover,non-targeted metabolomic analysis showed that MAG significantly promoted tryptophan metabolites,which was positively correlated with the MAG-enriched gut microbiota.The increased tryptophan metabolites could activate aryl hydrocarbon receptor(AhR)and relieved hepatic inflammation and immune response evidenced by the downregulated the gene expression levels of pro-inflammatory cytokines such as interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),and inte rleukin-6(IL-6)in the liver.The fecal microbiota transplantation(FMT)experiments further con firmed that the hepatoprotective effect is likely mediated by MAG-altered gut microbiota and their metabolites.Conclusions Magnolol can be an outstanding supplement for the preventio

关 键 词：Fecal microbiota transplantation FLHS Gut–liver axis Laying hens MAGNOLOL 

分 类 号：S858.31[农业科学—临床兽医学]