Effects of bacteriocin-producing Lactiplantibacillus plantarum on bacterial community and fermentation profile of whole-plant corn silage and its in vitro ruminal fermentation,microbiota,and CH_(4) emissions  

作　　者：Ziqian Li Samaila Usman Jiayao Zhang Yixin Zhang Rina Su Hu Chen Qiang Li Mengya Jia Tunde Adegoke Amole Xusheng Guo 

机构地区：[1]School of Life Sciences,Lanzhou University,Lanzhou 730000,P.R.China [2]Probiotics and Life Health Research Institute,Lanzhou University,Lanzhou 730000,P.R.China [3]International Livestock Research Institute(ILRI),IITA Campus PMB,Oyo Road,Ibadan 5320,Nigeria

出　　处：《Journal of Animal Science and Biotechnology》2024年第6期2625-2638,共14页畜牧与生物技术杂志(英文版)

基　　金：supported by the National Key R&D Program of China(grant number 2022YFD1301002)。

摘　　要：Background Silage is widely used to formulate dairy cattle rations,and the utilization of antibiotics and methane emissions are 2 major problems for a sustainable and environmentally beneficial ruminant production systems.Bacteriocin has received considerable attention because of its potential as an alternative to antibiotics in animal husbandry.However,the impact of bacteriocin-producing lactic acid bacteria on the microbiological conversion process of whole-plant corn silage and rumen fermentation remains limited.The purpose of this study was to assess the effect of 2 classⅡa bacteriocin-producing strains Lactiplantibacillusplontarum ATCC14917 and CICC24194 on bacterial community composition and ensiling profiles of whole-plant corn silage and its in vitro rumen fermentation,microbiota,and CH_(4) emissions.Results Both bacteriocin-producing strains increased the lactic acid concentration in silage fermented for 7 d,whereas the lowest lactic acid was observed in the ATCC14917 inoculated silage fermented for 90 d(P<0.05).The highest DM content was observed in the CICC24194 treatment(P<0.05),and the silages treated with both strains had the lowest DM loss(P<0.05).Bacteriocin-producing strains promoted the growth of Levilactobacillus brevis on d 60of ensiling.In addition,treatment with bacteriocin-producing strains increased the in vitro DM digestibility(P<0.05)and decreased the CH_(4) production(P<0.05).The results of random forest and clustering analyses at the genus level showed that ATCC14917 increased the relative abundance of the influential variable Bacillus compared to that in the control group,whereas CICC24194 decreased the relative abundance of the influential variable Ruminococcaceae UCG-005.The CICC24194 treatment had the lowest total bacterial,fungal,protozoan,and methanogen populations(P<0.05).Conclusions Both classⅡa bacteriocin-producing L.plantarum strains improved the fermentation quality of wholeplant corn silage by regulating the bacterial community composition during ensiling,with CICC2419

关 键 词：Bacteriocin-producing Lactiplantibacillus plantarum Fermentation Methane emissions MICROBIOTA RUMEN Whole-plant corn silage 

分 类 号：S816.53[农业科学—饲料科学]