五达颗粒与爱维莫潘对术后肠麻痹模型小鼠胃肠移行性复合运动的作用  

Comparison of the effects of Wuda granules and alvimopan on gastrointestinal migrating motor complex in postoperative ileus model mice

作　　者：陈其城 蒋志 陈志强 曹立幸 张义荣 CHEN Qi-cheng;JIANG Zhi;CHEN Zhi-qiang(Center of TCM Applications of Perioperative,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China)

机构地区：[1]广州中医药大学第二附属医院,广州510120 [2]广州中医药大学第三附属医院,广州510120

出　　处：《中国中西医结合外科杂志》2025年第1期111-116,共6页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金青年基金(82204716);国家区域中医(专科)诊疗中心建设专项资金资助(国中医药医政函[2018]205号广东省中医院外科);广州市科学技术局基础与应用基础研究基金资助项目(2023A03J0759、2024A03J0041);广东省科技厅-粤穗联合项目(2021A1515110773);广东省中医院临床研究专项(YN10101902);广东省中医药局项目(20211206、20225012)。

摘　　要：目的:比较五达颗粒和爱维莫潘对术后肠麻痹(POI)模型小鼠胃肠移行性复合运动(MMC)的作用。方法:在15只成年C56BL/6小鼠的胃窦、十二指肠浆膜表面植入微型应力传感器,随机分为对照组、POI-五达颗粒和POI-爱维莫潘组,每组各5只,比较不同剂量五达颗粒(0.75、1.5及3 g/kg)和爱维莫潘(0.25、0.5和1 mg/kg)对POI小鼠术后胃肠MMC的影响。结果:对照组小鼠胃窦、十二指肠出现典型的MMC,POI小鼠胃窦、十二指肠MMC周期紊乱,出现不规则收缩波,与对照组比较,POI小鼠收缩振幅、频率和振幅指数均下降,差异有统计学意义(P <0.001)。依次给予0.75、1.5及3 g/kg五达颗粒后,POI小鼠胃肠运动显著增强,可诱发高频率高振幅的MMCⅢ相样收缩,收缩频率、振幅和振幅指数与给药前基础值相比,均有明显增加,差异有统计学意义(P <0.001)。依次给予0.25、0.5和1 mg/kg爱维莫潘并未明显增强胃窦、十二指肠收缩能力,收缩频率、振幅和振幅指数与给药前相比无显著差异。结论:肠道操作导致小鼠胃肠MMC障碍,五达颗粒通过诱导MMCⅢ相样收缩而改善术后胃肠运动功能,爱维莫潘不是通过直接增强术后胃肠运动来治疗POI。Objective To observe the effect of Wuda granules and alvimopan on gastrointestinal migrating motor complex(MMC) movement in postoperative ileus model mice.Methods 15 adult C56BL/6 mice were implanted with miniature stress sensors on the surface of the gastric antrum and duodenal serosa,and randomly divided into a control group,POI-Wuda granules group,and POI-Avimopan group,with 5 mice in each group,to compare the effects of Wuda granules and Avimopan on postoperative gastrointestinal MMC motility in POI mice.Results The control group of mice showed typical MMC in the gastric antrum and duodenum.After intestinal manipulation,the MMC cycle in the gastric antrum and duodenum of mice was disrupted,and irregular contractile waves appeared.Compared with the control group,the amplitude,frequency,and amplitude index of contractions decreased(P <0.001).Sequential administration of Wuda granules(0.75,1.5 and 3 g/kg) induced high-frequency and high amplitude MMC Ⅲ phase like contractions,with significant increases in contraction frequency,amplitude,and amplitude index compared to the baseline values before administration(P <0.001).Sequential administration of alvimopan(0.25,0.5 and 1 mg/kg) did not significantly enhance gastric antral and duodenal contractions,with no significant differences in contraction frequency,amplitude,and amplitude index compared to before administration.Conclusion Intestinal manipulation leads to impaired gastrointestinal MMC motility in mice.Wuda granules improve postoperative gastrointestinal motility by inducing MMC phase Ⅲ contraction,while the effect of alvimopan in treating POI is not directly enhanced by postoperative gastrointestinal motility.

关 键 词：移行性复合运动 胃肠动力 五达颗粒 爱维莫潘 

分 类 号：R656[医药卫生—外科学] R269[医药卫生—临床医学]